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Anti-IMPA2 antibody (ab73076)

  • Datasheet
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Western blot - Anti-IMPA2 antibody (ab73076)
  • Immunocytochemistry/ Immunofluorescence - Anti-IMPA2 antibody (ab73076)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPA2 antibody (ab73076)

Key features and details

  • Rabbit polyclonal to IMPA2
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human IMPA2 protein (ab104020)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-IMPA2 antibody
  • Description

    Rabbit polyclonal to IMPA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human IMPA2 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab97986)

  • Positive control

    • This antibody gave a positive signal in Human Liver tissue lysate and HepG2 whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Phosphatases
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Lipid Phosphatases
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human IMPA2 protein (ab104020)

Applications

Our Abpromise guarantee covers the use of ab73076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).

Target

  • Function

    Can use myo-inositol monophosphates, scylloinositol 1,4-diphosphate, glucose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. Has been implicated as the pharmacological target for lithium Li(+) action in brain.
  • Pathway

    Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
  • Sequence similarities

    Belongs to the inositol monophosphatase family.
  • Target information above from: UniProt accession O14732 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3613 Human
    • Entrez Gene: 114663 Mouse
    • Entrez Gene: 282636 Rat
    • Omim: 605922 Human
    • SwissProt: O14732 Human
    • SwissProt: Q91UZ5 Mouse
    • SwissProt: Q8CIN7 Rat
    • Unigene: 723890 Human
    • Unigene: 726124 Human
    • Unigene: 34079 Mouse
    • Unigene: 441590 Mouse
    • Unigene: 856 Rat
    see all
  • Alternative names

    • IMP 2 antibody
    • IMP A2 antibody
    • IMP.18P antibody
    • IMP2 antibody
    • IMPA 2 antibody
    • Impa2 antibody
    • IMPA2_HUMAN antibody
    • IMPase 2 antibody
    • Inosine monophosphatase 2 antibody
    • Inositol 1 antibody
    • Inositol 1(or 4) monophosphatase 2 antibody
    • Inositol monophosphatase 2 antibody
    • Inositol monophosphatase 2 variant 1 antibody
    • Inositol monophosphatase 2 variant 2 antibody
    • Inositol(myo) 1(or 4) monophosphatase 2 antibody
    • Inositol-1(or 4)-monophosphatase 2 antibody
    • Myo inositol monophosphatase A2 antibody
    • Myo-inositol monophosphatase A2 antibody
    see all

Images

  • Western blot - Anti-IMPA2 antibody (ab73076)
    Western blot - Anti-IMPA2 antibody (ab73076)
    All lanes : Anti-IMPA2 antibody (ab73076) at 1/250 dilution

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 170 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    The 34 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human Inositol monophosphatase 2 (IMPA2)
  • Immunocytochemistry/ Immunofluorescence - Anti-IMPA2 antibody (ab73076)
    Immunocytochemistry/ Immunofluorescence - Anti-IMPA2 antibody (ab73076)
    ICC/IF image of ab73076 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73706, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPA2 antibody (ab73076)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPA2 antibody (ab73076)
    IHC image of IMPA2 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73076, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab73076? Please let us know so that we can cite the reference in this datasheet.

    ab73076 has not yet been referenced specifically in any publications.

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