Key features and details
- Rabbit polyclonal to IMPACT
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-IMPACT antibody
See all IMPACT primary antibodies
DescriptionRabbit polyclonal to IMPACT
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant fragment corresponding to Human IMPACT aa 41-320.
ISDDIDDPKWTLCLQVMLPNEYPGTAPPIYQLNAPWLKGQERADLSNSLE EIYIQNIGESILYLWVEKIRDVLIQKSQMTEPGPDVKKKTEEEDVECEDD LILACQPESSLKALDFDISETRTEVEVEELPPIDHGIPITDRRSTFQAHL APVVCPKQVKMVLSKLYENKKIASATHNIYAYRIYCEDKQTFLQDCEDDG ETAAGGRLLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILV EKNYTNSPEESSKALGKNKKVRKDKKRNEH
Database link: Q9P2X3
- WB: HeLa and HL-60 whole cell lysates. IHC-P: Human endometrial cancer and kidney tissue.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235323 in the following tested applications.
|WB||1/1000 - 1/5000. Predicted molecular weight: 36 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionTranslational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis.
Tissue specificityWidely expressed. Expressed at high level in brain.
Sequence similaritiesBelongs to the IMPACT family.
Contains 1 RWD domain.
- Information by UniProt
- IMPACT antibody
- Impact homolog (mouse) antibody
- Impact homolog antibody
All lanes : Anti-IMPACT antibody (ab235323) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa
Paraffin-embedded human endometrial cancer tissue stained for IMPACT using ab235323 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for IMPACT using ab235323 at 1/100 dilution in immunohistochemical analysis.
ab235323 has not yet been referenced specifically in any publications.