Overview

  • Product name

    Anti-IMPDH1 antibody [EPR9678(B)]
    See all IMPDH1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9678(B)] to IMPDH1
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 92% homology with IPMDH2. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IMPDH1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, K562, and Raji cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
    • Tissue specificity

      IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
    • Pathway

      Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
    • Involvement in disease

      Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant.
    • Sequence similarities

      Belongs to the IMPDH/GMPR family.
      Contains 2 CBS domains.
    • Information by UniProt
    • Database links

    • Alternative names

      • IMDH1_HUMAN antibody
      • IMP (inosine monophosphate) dehydrogenase 1 antibody
      • IMP dehydrogenase 1 antibody
      • IMPD 1 antibody
      • IMPD antibody
      • IMPD1 antibody
      • IMPDH 1 antibody
      • IMPDH I antibody
      • IMPDH-I antibody
      • Impdh1 antibody
      • Inosine 5' monophosphate dehydrogenase 1 antibody
      • Inosine monophosphate dehydrogenase 1 antibody
      • Inosine-5''-monophosphate dehydrogenase 1 antibody
      • LCA11 antibody
      • RP10 antibody
      • sWSS2608 antibody
      see all

    Images

    • All lanes : Anti-IMPDH1 antibody [EPR9678(B)] (ab137120) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    References

    This product has been referenced in:

    • Valvezan AJ  et al. mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability. Cancer Cell 32:624-638.e5 (2017). Read more (PubMed: 29056426) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137120.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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