Product nameAnti-IMPDH1 antibody [EPR9679(B)]
See all IMPDH1 primary antibodies
DescriptionRabbit monoclonal [EPR9679(B)] to IMPDH1
SpecificityThe immunogen used for this product shares 86% homology with IPMDH2. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human IMPDH1 aa 450-550 (C terminal). The exact sequence is proprietary.
- K562 cells; HeLa, Jurkat and K562 cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 55 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
Tissue specificityIMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
PathwayPurine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Involvement in diseaseDefects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant.
Sequence similaritiesBelongs to the IMPDH/GMPR family.
Contains 2 CBS domains.
- Information by UniProt
- IMDH1_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 1 antibody
- IMP dehydrogenase 1 antibody
Flow cytometric analysis of permeabilized K562 cells labelling IMPDH1 with ab137112 (red) at 1/500 dilution or a rabbit IgG (negative) (green).
All lanes : Anti-IMPDH1 antibody [EPR9679(B)] (ab137112) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
ab137112 has not yet been referenced specifically in any publications.