• Product name
  • Description
    Rabbit polyclonal to IMPDH2
  • Host species
  • Specificity
    The immunogen used to raise this antibody shares 81% homology with Human IPMDH1. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human IMPDH2 aa 467-514 (C terminal).


  • Positive control
    • HepG2 cell lysate.



Our Abpromise guarantee covers the use of ab75790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
  • Tissue specificity
    IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
  • Pathway
    Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
  • Sequence similarities
    Belongs to the IMPDH/GMPR family.
    Contains 2 CBS domains.
  • Post-translational
    The N-terminus is blocked.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMDH2_HUMAN antibody
    • IMP (inosine monophosphate) dehydrogenase 2 antibody
    • IMP dehydrogenase 2 antibody
    • IMP oxireductase 2 antibody
    • IMPD 2 antibody
    • IMPD2 antibody
    • IMPDH 2 antibody
    • IMPDH II antibody
    • IMPDH-II antibody
    • Impdh2 antibody
    • Inosine 5' monophosphate dehydrogenase 2 antibody
    • Inosine 5' phosphate dehydrogenase 2 antibody
    • Inosine monophosphate dehydrogenase type II antibody
    • Inosine-5''-monophosphate dehydrogenase 2 antibody
    see all


  • Anti-IMPDH2 antibody (ab75790) at 1 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • IHC image of ab75790 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75790, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab75790 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75790, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab75790 staining IMPDH2 in HEp-2 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody against IMPDH2 (red) and nuclei were stained with DAPI (blue).


This product has been referenced in:
  • Noble JW  et al. Loukoumasomes Are Distinct Subcellular Structures from Rods and Rings and Are Structurally Associated with MAP2 and the Nuclear Envelope in Retinal Cells. PLoS One 11:e0165162 (2016). ICC/IF ; Human . Read more (PubMed: 27798680) »
  • Fromm-Dornieden C  et al. Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. BMC Mol Biol 13:9 (2012). WB . Read more (PubMed: 22436005) »

See all 3 Publications for this product

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