Product nameAnti-IMPDH2 antibody [EPR8364(B)]
See all IMPDH2 primary antibodies
DescriptionRabbit monoclonal [EPR8364(B)] to IMPDH2
SpecificityThe immunogen used to raise this antibody shares 79% homology with Human IMPDH1. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human IMPDH2 aa 100-200. The exact sequence is proprietary.
- K562, HepG2, HeLa and NCI-H460 cell lysates; Human colon tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.20 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 56 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionRate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
Tissue specificityIMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
PathwayPurine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Sequence similaritiesBelongs to the IMPDH/GMPR family.
Contains 2 CBS domains.
modificationsThe N-terminus is blocked.
- Information by UniProt
- IMDH2_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 2 antibody
- IMP dehydrogenase 2 antibody
All lanes : Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NCI-H460 cell lysate
Predicted band size: 56 kDa
ab131158 staining IMPDH2 in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IMPDH2 with ab131158 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Rother M et al. Combined Human Genome-wide RNAi and Metabolite Analyses Identify IMPDH as a Host-Directed Target against Chlamydia Infection. Cell Host Microbe 23:661-671.e8 (2018). Read more (PubMed: 29706504) »
- Huang F et al. Inosine Monophosphate Dehydrogenase Dependence in a Subset of Small Cell Lung Cancers. Cell Metab 28:369-382.e5 (2018). Read more (PubMed: 30043754) »