Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8364(B)] to IMPDH2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-IMPDH2 antibody [EPR8364(B)]
See all IMPDH2 primary antibodies
DescriptionRabbit monoclonal [EPR8364(B)] to IMPDH2
SpecificityThe immunogen used to raise this antibody shares 79% homology with Human IMPDH1. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human IMPDH2 aa 100-200. The exact sequence is proprietary.
- K562, HepG2, HeLa and NCI-H460 cell lysates; Human colon tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.20 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 56 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionRate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
Tissue specificityIMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
PathwayPurine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Sequence similaritiesBelongs to the IMPDH/GMPR family.
Contains 2 CBS domains.
modificationsThe N-terminus is blocked.
- Information by UniProt
- IMDH2_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 2 antibody
- IMP dehydrogenase 2 antibody
All lanes : Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NCI-H460 cell lysate
Predicted band size: 56 kDa
ab131158 staining IMPDH2 in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IMPDH2 with ab131158 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131158 has been referenced in 9 publications.
- Kofuji S et al. IMP dehydrogenase-2 drives aberrant nucleolar activity and promotes tumorigenesis in glioblastoma. Nat Cell Biol 21:1003-1014 (2019). PubMed: 31371825
- Wolfe K et al. Dynamic compartmentalization of purine nucleotide metabolic enzymes at leading edge in highly motile renal cell carcinoma. Biochem Biophys Res Commun 516:50-56 (2019). PubMed: 31196624
- Duan S et al. IMPDH2 promotes colorectal cancer progression through activation of the PI3K/AKT/mTOR and PI3K/AKT/FOXO1 signaling pathways. J Exp Clin Cancer Res 37:304 (2018). PubMed: 30518405
- Rother M et al. Combined Human Genome-wide RNAi and Metabolite Analyses Identify IMPDH as a Host-Directed Target against Chlamydia Infection. Cell Host Microbe 23:661-671.e8 (2018). PubMed: 29706504
- Huang F et al. Inosine Monophosphate Dehydrogenase Dependence in a Subset of Small Cell Lung Cancers. Cell Metab 28:369-382.e5 (2018). PubMed: 30043754
- Dang W et al. Inhibition of Calcineurin or IMP Dehydrogenase Exerts Moderate to Potent Antiviral Activity against Norovirus Replication. Antimicrob Agents Chemother 61:N/A (2017). PubMed: 28807916
- Ziegler CM et al. A proteomic survey of Junín virus interactions with human proteins reveals host factors required for arenavirus replication. J Virol N/A:N/A (2017). PubMed: 29187543
- Lin R et al. CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis. Mol Cell 68:198-209.e6 (2017). PubMed: 28985504
- Valvezan AJ et al. mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability. Cancer Cell 32:624-638.e5 (2017). PubMed: 29056426