Overview

  • Product name

    Anti-IMPDH2 antibody [EPR8364(B)]
    See all IMPDH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8364(B)] to IMPDH2
  • Host species

    Rabbit
  • Specificity

    The immunogen used to raise this antibody shares 79% homology with Human IMPDH1. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IMPDH2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • K562, HepG2, HeLa and NCI-H460 cell lysates; Human colon tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.

Target

  • Function

    Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
  • Tissue specificity

    IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
  • Pathway

    Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
  • Sequence similarities

    Belongs to the IMPDH/GMPR family.
    Contains 2 CBS domains.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Information by UniProt
  • Database links

  • Alternative names

    • IMDH2_HUMAN antibody
    • IMP (inosine monophosphate) dehydrogenase 2 antibody
    • IMP dehydrogenase 2 antibody
    • IMP oxireductase 2 antibody
    • IMPD 2 antibody
    • IMPD2 antibody
    • IMPDH 2 antibody
    • IMPDH II antibody
    • IMPDH-II antibody
    • Impdh2 antibody
    • Inosine 5' monophosphate dehydrogenase 2 antibody
    • Inosine 5' phosphate dehydrogenase 2 antibody
    • Inosine monophosphate dehydrogenase type II antibody
    • Inosine-5''-monophosphate dehydrogenase 2 antibody
    see all

Images

  • All lanes : Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : NCI-H460 cell lysate

    Predicted band size: 56 kDa

  • ab131158 staining IMPDH2 in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IMPDH2 with ab131158 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Rother M  et al. Combined Human Genome-wide RNAi and Metabolite Analyses Identify IMPDH as a Host-Directed Target against Chlamydia Infection. Cell Host Microbe 23:661-671.e8 (2018). Read more (PubMed: 29706504) »
  • Huang F  et al. Inosine Monophosphate Dehydrogenase Dependence in a Subset of Small Cell Lung Cancers. Cell Metab 28:369-382.e5 (2018). Read more (PubMed: 30043754) »
See all 7 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human cancer xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9
Permeabilization
No
Specification
Human cancer xenograft
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

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Verified customer

Submitted Jun 29 2015

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