Recombinant
RabMAb

Recombinant Anti-IMPDH2 antibody [EPR8364(B)] - BSA and Azide free (ab240046)

Overview

  • Product name

    Anti-IMPDH2 antibody [EPR8364(B)] - BSA and Azide free
    See all IMPDH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8364(B)] to IMPDH2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IMPDH2 aa 100-200. The exact sequence is proprietary.

  • General notes

    Ab240046 is the carrier-free version of ab131158. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240046 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.

Target

  • Function

    Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
  • Tissue specificity

    IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
  • Pathway

    Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
  • Sequence similarities

    Belongs to the IMPDH/GMPR family.
    Contains 2 CBS domains.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Information by UniProt
  • Database links

  • Alternative names

    • IMDH2_HUMAN antibody
    • IMP (inosine monophosphate) dehydrogenase 2 antibody
    • IMP dehydrogenase 2 antibody
    • IMP oxireductase 2 antibody
    • IMPD 2 antibody
    • IMPD2 antibody
    • IMPDH 2 antibody
    • IMPDH II antibody
    • IMPDH-II antibody
    • Impdh2 antibody
    • Inosine 5' monophosphate dehydrogenase 2 antibody
    • Inosine 5' phosphate dehydrogenase 2 antibody
    • Inosine monophosphate dehydrogenase type II antibody
    • Inosine-5''-monophosphate dehydrogenase 2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IMPDH2 with ab131158 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131158).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ab131158 staining IMPDH2 in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131158).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131158).

References

ab240046 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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