Overview

  • Product name

    Anti-IMPDH2 antibody [EPR8365(B)]
    See all IMPDH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8365(B)] to IMPDH2
  • Host species

    Rabbit
  • Specificity

    The immunogen used to raise this antibody shares 86% homology with Human IMPDH1. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IMPDH2 aa 500-600 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HepG2, A549, MCF7, HeLa and K562 cell lysates; Human breast carcinoma and kidney tissues; MCF7 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
    • Tissue specificity

      IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
    • Pathway

      Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
    • Sequence similarities

      Belongs to the IMPDH/GMPR family.
      Contains 2 CBS domains.
    • Post-translational
      modifications

      The N-terminus is blocked.
    • Information by UniProt
    • Database links

    • Alternative names

      • IMDH2_HUMAN antibody
      • IMP (inosine monophosphate) dehydrogenase 2 antibody
      • IMP dehydrogenase 2 antibody
      • IMP oxireductase 2 antibody
      • IMPD 2 antibody
      • IMPD2 antibody
      • IMPDH 2 antibody
      • IMPDH II antibody
      • IMPDH-II antibody
      • Impdh2 antibody
      • Inosine 5' monophosphate dehydrogenase 2 antibody
      • Inosine 5' phosphate dehydrogenase 2 antibody
      • Inosine monophosphate dehydrogenase type II antibody
      • Inosine-5''-monophosphate dehydrogenase 2 antibody
      see all

    Images

    • All lanes : Anti-IMPDH2 antibody [EPR8365(B)] (ab129165) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 56 kDa
      Observed band size: 56 kDa

    • Overlay histogram showing K562 cells stained with ab129165 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129165, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab129165, at 1/250 dilution, staining IMPDH2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab129165, at 1/250 dilution, staining IMPDH2 in MCF7 cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Anthony SA  et al. Reconstituted IMPDH polymers accommodate both catalytically active and inactive conformations. Mol Biol Cell N/A:N/A (2017). WB ; Human . Read more (PubMed: 28794265) »
    • Barfeld SJ  et al. Myc-dependent purine biosynthesis affects nucleolar stress and therapy response in prostate cancer. Oncotarget 6:12587-602 (2015). IHC-P ; Human . Read more (PubMed: 25869206) »
    See all 2 Publications for this product

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