Overview

  • Product name

  • Description

    Goat polyclonal to Importin 7
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: SSFNFGGPAPGMN, corresponding to C terminal amino acids 1026-1038 of Human Importin 7.

  • Positive control

    • Human brain lysate. This antibody gave a positive result when used on methanol fixed SW480 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration. PubMed: 21419860

Target

  • Function

    Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In vitro, mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
  • Sequence similarities

    Belongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ14581 antibody
    • Imp 7 antibody
    • Imp7 antibody
    • Importin-7 antibody
    • Importin7 antibody
    • IPO 7 antibody
    • IPO7 antibody
    • IPO7_HUMAN antibody
    • MGC138673 antibody
    • Ran binding protein 7 antibody
    • Ran-binding protein 7 antibody
    • RANBP 7 antibody
    • RanBP7 antibody
    see all

Images

  • ab15840 stained SW480 cells. The cells were 100% methanol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15840 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150133 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

References

This product has been referenced in:

See all 5 Publications for this product

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