Overview

  • Product name
    Anti-Importin 9/RANBP9 antibody
    See all Importin 9/RANBP9 primary antibodies
  • Description
    Rabbit polyclonal to Importin 9/RANBP9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Importin 9/RANBP9 aa 925-975. The exact sequence is proprietary.
    Database link: Q96P70

  • Positive control
    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 cell lysates. IP: HeLa cell lysate.
  • General notes

     This product was previously labelled as Importin 9

     

Properties

Applications

Our Abpromise guarantee covers the use of ab241209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 116 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
  • Sequence similarities
    Belongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Imp9 antibody
    • Imp9a antibody
    • Imp9b antibody
    • Importin-9 antibody
    • Importin-9a antibody
    • Ipo9 antibody
    • IPO9_HUMAN antibody
    • Ran-binding protein 9 antibody
    • RanBP9 antibody
    see all

Images

  • All lanes : Anti-Importin 9/RANBP9 antibody (ab241209) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 116 kDa


    Exposure time: 75 seconds


    Lysates prepared in NETN buffer.

  • Importin 9/RANBP9 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241209 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241209 at 0.4 µg/ml.

    Lane 1: ab241209 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysates prepared in NETN buffer.

References

ab241209 has not yet been referenced specifically in any publications.

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