Recombinant
Recombinant Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-Importin 9/RANBP9 antibody [EP1353Y]
See all Importin 9/RANBP9 primary antibodies -
DescriptionRabbit monoclonal [EP1353Y] to Importin 9/RANBP9
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide within Human Importin 9/RANBP9 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q96P70 -
Positive control
- Fetal brain lysate; human lymphoma tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Importin 9
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1353Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
- Anti-Importin 9/RANBP9 antibody [EP1353Y] (Phycoerythrin) (ab208995)
- Anti-Importin 9/RANBP9 antibody [EP1353Y] (Alexa Fluor® 647) (ab210416)
- Anti-Importin 9/RANBP9 antibody [EP1353Y] (Alexa Fluor® 568) (ab211211)
- Anti-Importin 9/RANBP9 antibody [EP1353Y] (Alexa Fluor® 488) (ab211370)
- Anti-Importin 9/RANBP9 antibody [EP1353Y] (Alexa Fluor® 594) (ab215093)
- Anti-Importin 9/RANBP9 antibody [EP1353Y] - BSA and Azide free (ab247321)
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab52605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa). | |
| IP | 1/20. | |
| Flow Cyt | 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/100 - 1/250. |
Target
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FunctionFunctions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
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Sequence similaritiesBelongs to the importin beta family.
Contains 1 importin N-terminal domain. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 55705 Human
- Entrez Gene: 226432 Mouse
- SwissProt: Q96P70 Human
- SwissProt: Q91YE6 Mouse
- Unigene: 596014 Human
- Unigene: 271642 Mouse
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Alternative names
- Imp9 antibody
- Imp9a antibody
- Imp9b antibody
see all
Images
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![Western blot - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)](https://www.abcam.com/ps/products/52/ab52605/Images/ab52605wb.jpg)
Western blot - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605) at 1/1000 dilution + Fetal brain lysate at 10 µg
Secondary
HRP-labelled goat anti-rabbit. at 1/2000 dilution
Predicted band size: 115 kDa -
![Flow Cytometry - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)](https://www.abcam.com/ps/products/52/ab52605/Images/ab52605-3-ab52605FC.jpg)
Flow Cytometry - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)Overlay histogram showing HeLa cells stained with ab52605 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52605, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)](https://www.abcam.com/ps/products/52/ab52605/Images/ab52605ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)Immunohistochemical staining of paraffin-embedded human lymphoma using ab52605 at a dilution of 1/50.
Datasheets and documents
References
This product has been referenced in:
- Xue J et al. Forkhead Box M1 Is Essential for Nuclear Localization of Glioma-associated Oncogene Homolog 1 in Glioblastoma Multiforme Cells by Promoting Importin-7 Expression. J Biol Chem 290:18662-70 (2015). Read more (PubMed: 26085085) »
- Belin BJ et al. DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-½ that promotes efficient DNA repair. [corrected]. Elife 4:e07735 (2015). Read more (PubMed: 26287480) »
