Product nameAnti-Importin 9/RANBP9 antibody [EP1353Y] (Phycoerythrin)
See all Importin 9/RANBP9 primary antibodies
DescriptionRabbit monoclonal [EP1353Y] to Importin 9/RANBP9 (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Importin 9/RANBP9 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q96P70
- Flow Cyt: HeLa cells.
This product was previously labelled as Importin 9
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
- Anti-Importin 9/RANBP9 antibody [EP1353Y] (Alexa Fluor® 647) (ab210416)
- Anti-Importin 9/RANBP9 antibody [EP1353Y] (Alexa Fluor® 568) (ab211211)
- Anti-Importin 9/RANBP9 antibody [EP1353Y] (Alexa Fluor® 488) (ab211370)
- Anti-Importin 9/RANBP9 antibody [EP1353Y] (Alexa Fluor® 594) (ab215093)
- Anti-Importin 9/RANBP9 antibody [EP1353Y] - BSA and Azide free (ab247321)
- Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)
Our Abpromise guarantee covers the use of ab208995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF
FunctionFunctions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
Sequence similaritiesBelongs to the importin beta family.
Contains 1 importin N-terminal domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Imp9 antibody
- Imp9a antibody
- Imp9b antibody
Overlay histogram showing HeLa cells stained with ab208995 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min at 22°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208995, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 at 22°C for 15 min used under the same conditions.
ab208995 has not yet been referenced specifically in any publications.