Recombinant Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
![Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.com/ps/products/124/ab124710/Images/ab124710-392195-anti-ipo9-epr1352-antibody-western-blot-wt-hela-ipo9-ko-hela-sk-br-3-cells.jpg "Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1352] to Importin 9/RANBP9
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Importin 9/RANBP9 antibody [EPR1352]
See all Importin 9/RANBP9 primary antibodies -
Description
Rabbit monoclonal [EPR1352] to Importin 9/RANBP9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
corresponding to Human Importin 9/RANBP9 aa 50-150.
Database link: Q96P70 -
Positive control
- HeLa, SKBR-3, C6, and NIH/3T3 cell lysates and Human brain tissue.
-
General notes
This product was previously labelled as Importin 9
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.29 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1352 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
|
|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 116 kDa. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6. -
Sequence similarities
Belongs to the importin beta family.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 55705 Human
- Entrez Gene: 226432 Mouse
- Entrez Gene: 304817 Rat
- SwissProt: Q96P70 Human
- SwissProt: Q91YE6 Mouse
- Unigene: 596014 Human
- Unigene: 271642 Mouse
- Unigene: 198415 Rat
-
Alternative names
- Imp9 antibody
- Imp9a antibody
- Imp9b antibody
see all
Images
-
Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : IPO9 knockout HeLa cell lysate
Lane 3 : SK-BR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 116 kDaLanes 1-3: Merged signal (red and green). Green - ab124710. Red - loading control, ab8245 observed at 36 kDa.
ab124710 was shown to react with IPO9 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265352 (knockout cell lysate ab257483) was used. Wild-type and IPO9 knockout samples were subjected to SDS-PAGE. ab124710 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.com/ps/products/124/ab124710/Images/ab124710-138511-anti-importin-9-antibody-epr1352-western-blot.jpg)
Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 116 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.com/ps/products/124/ab124710/Images/ab124710-138510-anti-importin-9-antibody-epr1352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)ab124710, at 1/500 to 1/1000, staining Impotin 9/RANBP9 in Human brain tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.com/ps/products/124/ab124710/Images/ab124710-138509-anti-importin-9-antibody-epr1352.jpg)
OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) -
![Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.com/ps/products/124/ab124710/Images/ab124710-5-benefits-of-recombinant-antibodies.png)
Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
Protocols
Datasheets and documents
References (2)
ab124710 has been referenced in 2 publications.
- Hirai Y et al. Intracellular dynamics of actin affects Borna disease virus replication in the nucleus. Virus Res 263:179-183 (2019). PubMed: 30769121
- Liu C et al. Nuclear Actin Polymerized by mDia2 Confines Centromere Movement during CENP-A Loading. iScience 9:314-327 (2018). PubMed: 30448731
