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    importin-9ranbp9-antibody-epr1352-ab124710.pdf

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Signal Transduction Protein Trafficking Nuclear Import / Export
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

  • Datasheet
  • SDS
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Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
  • Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
  • OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
  • Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1352] to Importin 9/RANBP9
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Human IPO9 (Importin 9/RANBP9) knockout HeLa cell lysate (ab257483)
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Anti-Peg3 antibody (ab99252)

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Overview

  • Product name

    Anti-Importin 9/RANBP9 antibody [EPR1352]
    See all Importin 9/RANBP9 primary antibodies
  • Description

    Rabbit monoclonal [EPR1352] to Importin 9/RANBP9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, SKBR-3, C6, and NIH/3T3 cell lysates and Human brain tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.29 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1352
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • Alternative Versions

    • Anti-Importin 9/RANBP9 antibody [EPR1352] - BSA and Azide free (ab247974)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 nuclear extract lysate (ab14874)
    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
  • Sequence similarities

    Belongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q96P70 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55705 Human
    • Entrez Gene: 226432 Mouse
    • Entrez Gene: 304817 Rat
    • SwissProt: Q96P70 Human
    • SwissProt: Q91YE6 Mouse
    • Unigene: 596014 Human
    • Unigene: 271642 Mouse
    • Alternative names

      • Imp9 antibody
      • Imp9a antibody
      • Imp9b antibody
      • Importin-9 antibody
      • Importin-9a antibody
      • Ipo9 antibody
      • IPO9_HUMAN antibody
      • Ran-binding protein 9 antibody
      • RanBP9 antibody
      see all

    Images

    • Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
      Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
      All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : IPO9 knockout HeLa cell lysate
      Lane 3 : SK-BR-3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 116 kDa



      Lanes 1-3: Merged signal (red and green). Green - ab124710. Red - loading control, ab8245 observed at 36 kDa. 

      ab124710 was shown to react with IPO9 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265352 (knockout cell lysate ab257483) was used. Wild-type and IPO9 knockout samples were subjected to SDS-PAGE. ab124710 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
      Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
      All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SKBR-3 cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 116 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

      ab124710, at 1/500 to 1/1000, staining Impotin 9/RANBP9 in Human brain tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
      OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
      Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab124710? Please let us know so that we can cite the reference in this datasheet.

    ab124710 has been referenced in 2 publications.

    • Hirai Y  et al. Intracellular dynamics of actin affects Borna disease virus replication in the nucleus. Virus Res 263:179-183 (2019). PubMed: 30769121
    • Liu C  et al. Nuclear Actin Polymerized by mDia2 Confines Centromere Movement during CENP-A Loading. iScience 9:314-327 (2018). PubMed: 30448731

    Customer reviews and Q&As

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