Product nameAnti-Importin 9/RANBP9 antibody [EPR1352]
See all Importin 9/RANBP9 primary antibodies
DescriptionRabbit monoclonal [EPR1352] to Importin 9/RANBP9
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human Importin 9/RANBP9 aa 50-150.
Database link: Q96P70
- HeLa, SKBR-3, C6, and NIH/3T3 cell lysates and Human brain tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.29 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 116 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/250 - 1/500.|
FunctionFunctions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
Sequence similaritiesBelongs to the importin beta family.
Contains 1 importin N-terminal domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Imp9 antibody
- Imp9a antibody
- Imp9b antibody
All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 116 kDa
ab124710, at 1/500 to 1/1000, staining Impotin 9/RANBP9 in Human brain tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Hirai Y et al. Intracellular dynamics of actin affects Borna disease virus replication in the nucleus. Virus Res 263:179-183 (2019). Read more (PubMed: 30769121) »
- Liu C et al. Nuclear Actin Polymerized by mDia2 Confines Centromere Movement during CENP-A Loading. iScience 9:314-327 (2018). Read more (PubMed: 30448731) »