Overview

  • Product name

    Anti-Importin4/Imp4 antibody [EPR13660-27]
    See all Importin4/Imp4 primary antibodies
  • Description

    Rabbit monoclonal [EPR13660-27] to Importin4/Imp4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Importin4/Imp4. The exact sequence is proprietary.
    Database link: Q8TEX9

  • Positive control

    • HepG2, HeLa, A549, human seminoma and PC-12 lysates. Human colon and testis tissue. A549 cells. Permeabilized A549 cells. Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900).
  • General notes

     This product was previously labelled as Importin4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 119 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.

Target

  • Function

    Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • Sequence similarities

    Belongs to the importin beta family.
    Contains 6 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Imp4 antibody
    • Imp4b antibody
    • Importin 4 antibody
    • Importin 4b antibody
    • Importin-4 antibody
    • Importin-4b antibody
    • Ipo4 antibody
    • IPO4_HUMAN antibody
    • Ran binding protein 4 antibody
    • Ran-binding protein 4 antibody
    • RanBP4 antibody
    see all

Images

  • All lanes : Anti-Importin4/Imp4 antibody [EPR13660-27] (ab181037) at 1/10000 dilution

    Lane 1 : HepG2 lysates
    Lane 2 : HeLa lysates
    Lane 3 : A549 lysates
    Lane 4 : Human seminoma lysates
    Lane 5 : PC-12 lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 119 kDa

  • Immunohistochemical analysis of paraffin-embedded human colon tissue staining Importin4/Imp4 using ab181037 at a 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue staining Importin4/Imp4 using ab181037 at a 1/100 dilution.

  • Immunofluorescent staining Importin4/Imp4 in A549 cells using ab181037 at a 1/100 dilution (red). DAPI nuclear staining (blue).

  • Flow cytometric analysis of permeabilized A549 cells using ab181037 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab181037 at a 1/10 dilution for IP.

References

This product has been referenced in:

  • Jing R  et al. Motifs in the amino-terminus of CENP-A are required for its accumulation within the nucleus and at the centromere. Oncotarget 8:40654-40667 (2017). Read more (PubMed: 28489565) »
See 1 Publication for this product

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