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    in-cell-elisa-ice-support-pack-wo-plates-ab111541.pdf

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In-Cell ELISA (ICE) Support Pack w/o plates (ab111541)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (1)

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In-Cell ELISA - ICE (In-Cell ELISA) Support Pack w/o plates (ab111541)

    Key features and details

    • Assay type: Cell-based (quantitative)
    • Sample type: Adherent cells, Suspension cells

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    Overview

    • Product name

      In-Cell ELISA (ICE) Support Pack w/o plates
      See all In cell elisa kits
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Cell-based (quantitative)
    • Assay duration

      Multiple steps standard assay
    • Product overview

      Note - This kit does not include cell culture plates. 96 well or 384 well plates which are appropriate for the requirements of your cell line must be purchased seperately. However an alternative support pack, ab111542 which contains five high quality 96-well microplates suitable for ICE is available.


      ab111541 is for use with suspension, apoptotic/detaching cells and adherent cell lines. The pack contains buffers and a protocol to perform ICE with Abcam ICE-validated antibodies. For an ICE assay, it is necessary to purchase both primary antibody(ies) and labeled secondary antibody(ies). Antibodies are sold separately, allowing customizing the target(s) of interest, method of detection and multiplexing.


      In-Cell ELISA uses quantitative immunocytochemistry to measure protein levels or post-translational modifications within cells. The cells are fixed to the bottom of a coated 96-well plate (not provided). Targets of interest are detected by primary antibodies, which are in turn quantified with labeled secondary antibody(ies). Abcam offers highly-specific, well-characterized primary antibodies, IRDye®- or HRP-labeled anti-mouse and anti-rabbit secondary antibodies, as well as IRDye®-labeled isotype specific anti-mouse antibodies. By combining antibodies of different species or isotype and appropriate IR-labeled secondary antibodies, two color multiplexing can be achieved in the 800/700 channels. IR imaging and quantification is performed using a LI-COR® Odyssey® or Aerius® system. HRP-labeled complexes are developed and quantified colorimerically using spectrophotometer.


      Two protocols are available when using this kit. Protocol (1) suspension cells and cells likely to detach under experimental conditions (for example, adherent cells undergoing apoptosis readily detach from a culture plate). Protocol (2) - A second protocol is available for normal adherent cells. These protocols can be used with any of Abcam's ICE-validated antibodies. Specific scientific information, background and working concentration for each antibody are detailed in each antibody's corresponding datasheet.

    • Notes

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 5 x 96 tests
      100X Triton X-100 1 x 1.25ml
      10X Blocking Solution 1 x 40ml
      10X Phosphate Buffered Saline (PBS) 1 x 250ml
      400X Tween-20 1 x 4ml
      Janus Green Stain 1 x 30ml

    Associated products

    • Related Products

      • MitoBiogenesis™ In-Cell ELISA Kit (IR) (ab110216)
      • MitoBiogenesis™ In-Cell ELISA Kit (Colorimetric) (ab110217)

    Images

    • In-Cell ELISA - ICE (In-Cell ELISA) Support Pack w/o plates (ab111541)
      In-Cell ELISA - ICE (In-Cell ELISA) Support Pack w/o plates (ab111541)
      Cells are grown to ~80% confluency in a 96- or 384-well plate, a drug/other treatment is applied to stimulate a cellular response. The cells are then fixed and permeabilized, effectively "freezing" them. Primary antibodies are then added which bind to their intended targets within the mitochondria or other subcellular compartment. After incubation, the unbound primary antibodies are washed away and secondary antibodies are added. These secondaries are conjugated to either IRDyes® or to an enzyme label (HRP or AP) for the colorimetric versions of the assays. Unbound secondaries are washed away, reaction buffer is added for the colorimetric assays, and the signal is read on a suitable instrument for the kit type.
      » In-cell ELISA diagram in PDF format

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab111541? Please let us know so that we can cite the reference in this datasheet.

    ab111541 has been referenced in 1 publication.

    • Shi H  et al. Endothelium-protective, histone-neutralizing properties of the polyanionic agent defibrotide. JCI Insight 6:N/A (2021). PubMed: 34264868

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question


    in Ihrem MitoSciences In-Cell ELISA Support Pack - Suspension Cell
    Protocol wird beschrieben, wie man Suspensionszellen in einer 96-well
    Platte fixieren kann. In dem dazugehörigen Kit sind schon einige Assay
    plates enthalten. Nun ist meine Frage, ob dieses Protokoll mit beliebigen
    96-well Platten funktioniert oder ob die dazugehörigen Platten speziell
    beschichtet sind und wenn ja, mit was?
    Schonmal im Voraus vielen Dank für Ihre Hilfe.
    Mit freundlichen Grüßen,

    Read More

    Abcam community

    Verified customer

    Asked on May 13 2014

    Answer



    Ich möchte Ihnen hiermit bestätigen welche Platten wir für den In-Cell ELISA empfehlen:

    Schwarze Platte mit durchsichtigem Boden für Fluoreszenz-Anwendungen

    Wir empfehlen Platten, die mit Aminen (Aminogruppen) beschichtet sind, da diese ein gleichmäßigeres Aussähen ermöglichen.

    Read More

    Anja Hoffmann

    Abcam Scientific Support

    Answered on May 13 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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