Key features and details
- Detection method: Flow cytometry-fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 3 hr
- Sample type: Cell culture extracts, Tissue sections
Product nameIn situ BrdU DNA Fragmentation Assay Kit
See all DNA fragmentation kits
Detection methodFlow cytometry-fluorescent
Sample typeCell culture extracts, Tissue sections
Assay time3h 00m
Species reactivityReacts with: Mammals
InSitu BrdU DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.
PlatformFlow cytometer, Fluorescence microscope
Storage instructionsPlease refer to protocols.
Components Identifier 60 tests Anti-BdU-FITC Antibody Orange 1 x 0.3ml Br dUTP Violet 1 x 0.48ml Negative Control Cells Natural 1 x 5ml PI/Nase Staining buffer Amber 1 x 30ml Positive Control Cells Brown 1 x 5ml Reaction buffer Green 1 x 0.6ml Rinse buffer Red 1 x 120ml TdT Enzymes Yellow 1 x 45µl Wash buffer Blue 1 x 120ml
RelevanceInternucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
ab66098 has not yet been referenced specifically in any publications.