In situ BrdU DNA Fragmentation Assay Kit (ab66098)
Key features and details
- Detection method: Flow cytometry-fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 3 hr
- Sample type: Cell culture extracts, Tissue sections
Overview
-
Product name
In situ BrdU DNA Fragmentation Assay Kit
See all DNA fragmentation kits -
Detection method
Flow cytometry-fluorescent -
Sample type
Cell culture extracts, Tissue sections -
Assay time
3h 00m -
Species reactivity
Reacts with: Mammals -
Product overview
InSitu BrdU DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.
-
Platform
Flow cytometer, Fluorescence microscope
Properties
-
Storage instructions
Please refer to protocols. -
Components Identifier 60 tests Anti-BdU-FITC Antibody Orange 1 x 0.3ml Br dUTP Violet 1 x 0.48ml Negative Control Cells Natural 1 x 5ml PI/Nase Staining buffer Amber 1 x 30ml Positive Control Cells Brown 1 x 5ml Reaction buffer Green 1 x 0.6ml Rinse buffer Red 1 x 120ml TdT Enzymes Yellow 1 x 45µl Wash buffer Blue 1 x 120ml -
Research areas
-
Relevance
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab66098 has not yet been referenced specifically in any publications.