Anti-INCENP antibody (ab12183)
Key features and details
- Rabbit polyclonal to INCENP
- Suitable for: IP, ICC, WB, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Human, Xenopus laevis
- Isotype: IgG
Overview
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Product name
Anti-INCENP antibody
See all INCENP primary antibodies -
Description
Rabbit polyclonal to INCENP -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC, WB, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Xenopus laevis
Predicted to work with: Chicken -
Immunogen
Synthetic peptide:
SKPRYHKRTSSAVWNSP
(with N-terminal added cysteine) conjugated to KLH, corresponding to amino acids 884-901 of Human INCENP. -
General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. There is some debate about whether this antibody is suitable for use in IF and we would be most interested to hear from researchers who test this antibody in this application. The antibody has been reported to be suitable for use in IF at a dilution of 1/100 using HeLa cells (see image below). However, one of our customers used this product in HeLa cells and reported an unusual staining pattern - please see the enquiries section of the online datasheet for further details.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC | (2) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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ICC/IF | (3) |
Use at an assay dependent concentration.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
|
Notes |
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IP
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1. -
Sequence similarities
Belongs to the INCENP family. -
Cellular localization
Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 396270 Chicken
- Entrez Gene: 3619 Human
- Entrez Gene: 16319 Mouse
- Omim: 604411 Human
- SwissProt: Q9NQS7 Human
- SwissProt: Q9WU62 Mouse
- Unigene: 142179 Human
- Unigene: 29755 Mouse
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Alternative names
- binds and activates aurora B and C in vivo and in vitro antibody
- Chromosomal passenger protein antibody
- INCE_HUMAN antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab12183 has been referenced in 22 publications.
- Santos-Barriopedro I et al. Off-the-shelf proximity biotinylation using ProtA-TurboID. Nat Protoc 18:36-57 (2023). PubMed: 36224470
- Hong J et al. Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression. J Ginseng Res 46:481-488 (2022). PubMed: 35600766
- Park M et al. A novel synthetic microtubule inhibitor exerts antiproliferative effects in multidrug resistant cancer cells and cancer stem cells. Sci Rep 11:10822 (2021). PubMed: 34031528
- Tsunematsu T et al. APC/CCdh1 is required for the termination of chromosomal passenger complex activity upon mitotic exit. J Cell Sci 133:N/A (2020). PubMed: 32934012
- Kim S et al. PRMT6-mediated H3R2me2a guides Aurora B to chromosome arms for proper chromosome segregation. Nat Commun 11:612 (2020). PubMed: 32001712