Other product details
Host species: Goat
Primary antibody (in Abdil) incubated OVERNIGHT (1 hour didn't work) at room temp.
Other details above.
Cells co-stained with mouse anti-tubulin (secondary is Anti-Mouse Alexa 488) and ACA (Human anti centromere marker, secondary is Anti-Human Cy5).
Cells also stained with DAPI.
Figure shows projected images of dividing cells (prophase, prometaphase, metaphase, anaphase, telophase). Top panels show ab12183, DNA and microtubule stains. Lower panels are the same cells, showing a subset of optical sections at higher magnification. ACA (geen) stains centromeres and ab12183 (red) stains the inner centromere in prophase, prometaphase, and metaphase. The ab12183 channel has been linerally scaled to reduce the background staining associated with this antibody.
Dr. William Moore
Submitted Nov 30 2005