CyGEL™ (ab109204)
Overview
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Product name
CyGEL™ -
Tested applications
Suitable for: FM, ICC/IFmore details -
General notes
CyGEL™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling.
CyGEL™ has many applications in imaging and cell-based screening.
Key features of CyGEL™ include:- Convenient immobilization of non-adherent cells.
- Controllable and rapid transition from liquid to gel, which allows quick preparation of live cells for gel mounting.
- Optically clear with low autofluorescence.
- Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).
CyGEL™ is recommended for mounting live de-adherent/ non-adherent cells for microscopy, or mounting live C. elegans and parasites (e.g. leishmania, trypanosomes), which can be recovered afterwards. CyGEL™ is provided as concentrated non-buffered form. Add the concentrated PBS supplement (provided) to prime CyGEL™ prior use.
CyGEL™ is supplied as 8 vials, each containing 500µl, and 1 vial of 40xPBS.
For imaging experiments longer than 2 hours, we recommend using CyGEL Sustain™ (ab109205).
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. Do not freeze. -
Storage buffer
Constituent: 30% Oxirane methyl polymer with oxirane -
Concentration information loading...
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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FM |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
Please refer to the Protocol Booklet for detailed information about the use of CyGEL. |
Notes |
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FM
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. Please refer to the Protocol Booklet for detailed information about the use of CyGEL. |
Images
Datasheets and documents
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Datasheet download
References (0)
ab109204 has not yet been referenced specifically in any publications.