20x TBS-T with Tween 20 (ab64204)
Overview
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Product name
20x TBS-T with Tween 20
See all TBS reagents -
Tested applications
Suitable for: IHC-Pmore details -
General notes
Tris Buffered Saline (TBS) wash buffer with Tween 20 for use during immunolabeling techniques such as IHC.
The salts in 20x solution are at a very high concentration. It has been found that at temperatures below 44 degrees F (6 degrees C), the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution.
Properties
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Form
Liquid -
Storage instructions
Store at room temperature. -
Storage buffer
pH: 7.2
Constituents: 99% Tris buffered saline (x20), 1% Tween
20x Concentrated -
Concentration information loading...
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Research areas
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Relevance
TBS is a solution of Tris Buffered Saline for use with immunolabeling techniques as a wash buffer. Tween 20 is sometimes added to promote more effective washings, resulting in decreased non-specific background staining. Tween 20 is also known to promote reagent spreading in automated staining methodologies. -
Alternative names
- Tris buffered saline
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent dilution.
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Notes |
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IHC-P
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab64204 has been referenced in 1 publication.
- Jiang Y et al. MAP30 promotes apoptosis of U251 and U87 cells by suppressing the LGR5 and Wnt/ß-catenin signaling pathway, and enhancing Smac expression. Oncol Lett 15:5833-5840 (2018). PubMed: 29556310