Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
Key features and details
- Goat polyclonal to alpha Defensin 1 + 2 + 3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Defensin 1 + 2 + 3 antibody -
Description
Goat polyclonal to alpha Defensin 1 + 2 + 3 -
Host species
Goat -
Specificity
ab99504 is expected to equally recognize alpha Defensin 1 precursor and alpha Defensin 3 precursor. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
C-DESLAPKHPGSRKN
, corresponding to internal sequence amino acids 50-63 of Human alpha Defensin 1 + 2 + 3 (NP_004075.1; NP_005208.1). -
Positive control
- WB: Human spleen lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 10 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 10 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Relevance
Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 1667 Human
- Omim: 125220 Human
- SwissProt: P59665 Human
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Alternative names
- DEF1 antibody
- DEF3 antibody
- DEFA1 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab99504 has not yet been referenced specifically in any publications.