EpiSeeker Sample Preparation kits are the first step to your successful experiment.
More often than not, your starting material is the key to a good result: if you have a good sample to analyze, you know your experiment is likely to succeed.
So why risk your experiment using sub-optimal techniques to obtain your sample? Our new range of sample preparation kits has been optimized to be used with our EpiSeeker range of Epigenetic kits, making sure you start your analysis with the optimal preparation.
Which product to use?
It can be difficult sometimes to know which product is more suitable for your experiment.
The table below gives a summary of the characteristics of the kits available for sample preparation and for which application is most suitable.
| EpiSeeker Nuclear Extraction kit | EpiSeeker Nuclear (NA-free) Extraction Kit | EpiSeeker Whole Cell Extraction Kit | EpiSeeker Histone Extraction kit | EpiSeeker Chromatin Extraction Kit | |
|---|---|---|---|---|---|
| AbID | ab113474 | ab113477 | ab113475 | ab113476 | ab117152 |
| Application(s) | Enzyme assay activity Protein detection | Protein detection
| Enzyme activity assay Protein detection | Histone detection
| Chromatin IP in vitro DNA-protein binding assays nuclear enzyme assays |
| Sample input | cells / tissue | cells / tissue | cells | cells / tissue | cells / tissue |
| Sample amount* | cells: 2-5 million / 80%-90% confluent tissue: variable | cells: 2-5 million / 80%-90% confluent tissue: variable | cells: 2-5 million / 80%-90% confluent | cells: 2-5 million / 80%-90% confluent tissue: 10 mg | cells: 0.1-10 million tissue: 50 - 200 mg |
| Processing time | ≤ 60 min | ≤ 60 min | ≤ 45 min | ≤ 60 min | ≤ 60 min |
| Sample yield | 5µg prot /10mg tissue - 1 million cells | 5µg prot /10mg tissue - 1 million cells | 10µg prot /10mg tissue - 1 million cells | 0.4µg prot /100mg tissue - 10 million cells | 4µg prot /50mg tissue - 10 million cells |
| Extracted sample | Nuclear protein | Nucleic acid-free nuclear protein | Whole cell protein | Histone protein | Chromatin (DNA & bound protein) |
| Active enzyme? | Yes | No | Yes | No | Yes |
* Amount of sample necessary will vary depending on the type of cells or tissue. Please refer to specific product protocol for detailed information.
Which samples to use?
The EpiSeeker Sample Preparation kits can be used for extractions in a broad range of samples, such as:
- cultured cells (suspension or adherent)
- fresh or frozen tissue
- whole blood
- urine
- buccal cells / swabs
- other fluids (semen)
Related products
For more information on additional epigenetic kits, please visit our EpiSeeker page.