Bisulfite DNA modification
Need to perform bisulfite conversion on your DNA sample? Look no further. Our efficient bisulfite modification kits are the fastest available:
- Quick: less than 3 hours processing time
- High yield and > 99.9 % conversion efficiency
- Eluted DNA is ready for direct processing: sequencing, PCR, etc.,...
- Wide range of sample types - including cells and tissue
What is bisulfite DNA modification?
Sodium bisulfite modification, also known as bisulfite conversion, is one of the most useful tools for analyzing methylation of cytosine residues at position 5.
This method is based on treating DNA with sodium bisulfite in order to determine its methylation pattern.
Treatment of DNA with bisulfite (sulphonation) leads to the deamination of cytosine residues and converts them to uracil, while 5-methylcytosine residues remain the same (Figure 1).
Bisulfite-modified DNA is generally analyzed by PCR, which can be performed under methylation- or non-methylation-specific conditions. The treatment will generate specific changes in the DNA sequence that will depend on the methylation status of individual cytosine residues, providing single-nucleotide resolution information about the methylation status of a DNA region: cytosine residues that have been converted to uracil will be detected as thymine residues whereas methylated cytosine residues will be detected as cytosines (Figure 2).
The analysis of the difference between the amount of cytosines and thymines on untreated / treated sample will reflect the methylation status.
The table below gives a summary of the characteristics of the different kits available to perform bisulfite analysis:
|EpiSeeker methylated control DNA kit||EpiSeeker DNA purification & modification kit||EpiSeeker Bisulfite modification kit - whole cell||EpiSeeker Fast DNA modification kit|
|What is it for?|| |
Methylation-positive control for bisulfite conversion treatments
DNA isolation and bisulfite conversion from sample (in two separate steps)
Direct bisulfite conversion from cells or tissue (no DNA isolation step)
Bisulfite conversion of purified DNA
|Sample input|| |
serum, plasma and body fluids
cultured cells, tissue samples, embryonic cells / oocytes
|Minimum sample amount||100 pg DNA||500 cells / ≤ 1 µl blood||≤ 100 cells / 1 µg tissue||0.2 ng DNA (50 cells)|
|Bisulfite processing time||1h 30 min||1h 30 min||1h 30 min||20 min|
|Total processing time||< 2 h||≤ 2h 30 min||< 3 h||30 min|
|Elution volume||8 - 20 µl||8 - 20 µl||8 - 20 µl||8 - 20 µl|
|Bisulfite DNA recovery||75%||> 75%||> 85%||>90%|