Stem cell differentiation protocol

Human embryonic stem cells to hepatic endoderm

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The ability to produce a homogenous population of functional cells in large quantities is one of the major challenges for stem cell biology. This is a robust 17 day differentiation protocol to direct human embryonic stem cells (hESCs) towards hepatocyte-like cells.


It contains detailed information about:

  • Preparation of chemical stocks
  • Coating of culture plasticware
  • Preparation of media
  • Priming hESCs to definitive endoderm

Edited protocol reproduced with kind permission of David Hay and the Journal of Visualized Experiments


Download the differentiation protocol.


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