The golgi apparatus or complex is involved in the processing and sorting of proteins and lipids between cellular destinations, particularly receiving proteins from the ER. It is composed of a series of flat membranous sacs, packed together. This membrane stack is highly polar, varying both in thickness and membrane protein contents. The cis face receives vesicles from the ER and their contents move through the golgi, eventually budding off from the trans face. However, this complex is highly dynamic - fusion also occurs at the trans face and it is thought that the backward budding and fusion of vesicles facilitates the recycling of membrane and receptors.
58K protein - 58K Golgi protein antibody (ab5820)
58K protein antibodies are frequently used markers for the Golgi complex. The 58K protein was first identified as being associated with the cytoplasmic surface of the Golgi apparatus in vivo and having a role in binding microtubules. It has since been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.
AP1G1 - AP1G1 antibody (ab3706)
AP1G1 is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
GM130 is a peripheral cytoplasmic protein that is tightly bound to Golgi membranes and part of a larger oligomeric complex. Its protein sequence suggests that GM130 is an extended rod-like protein with coiled-coil domains. Immunofluorescence has shown that it colocalises with the cis-golgi marker syntaxin 5 and this was confirmed by electron microscopy. It is thought that GM130 is part of a cis-Golgi matrix and has a role in maintaining cis-Golgi structure.
GOLGA1 - GOLGA1 antibody [CDFX] (ab15845)
GOLGA1 is a member of the golgin family of proteins, which are localized to the Golgi. GOLGA1 is associated with Sjogren's syndrome.
Mannosidase II - Mannosidase II antibody (ab12277)
Mannosidase II is a 135kD protein located on the luminal side of the Golgi membranes. It is useful as an immunocytochemical marker for the Golgi apparatus as well as for studying the fate of the Golgi complex in dividing cells.
TGN 38 - TGN38 antibody [2F7.1] (ab2809)
The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways. TGN 38 and TGN 41, an isoform of the former, are integral membrane proteins which are predominantly localized to the TGN in NRK cells but which can also be observed at the cell surface. Complexes which include TGN 38, low molecular weight G proteins and p62 are required for the formation of TGN-derived vesicles. This requirement has lead people to believe that TGN 38 is important in the trafficking of proteins from the TGN to the cell surface. Brefeldin A causes the contents of the Golgi stack to be redistributed into the endoplasmic reticulum while the TGN collapse upon the microtubule organising centre. This makes immunofluorescence detection of TGN 38 following brefeldin A treatment a useful tool for the discrimination of the TGN from the Golgi stack.