Anti-Borealin/CDCA8 antibody (ab67126)
Key features and details
- Mouse polyclonal to Borealin/CDCA8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Borealin/CDCA8 antibody
See all Borealin/CDCA8 primary antibodies -
Description
Mouse polyclonal to Borealin/CDCA8 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native Borealin/CDCA8 (purified) (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
Target
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Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. -
Sequence similarities
Belongs to the borealin family. -
Developmental stage
Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase. -
Domain
The C-terminal region (aa 207-280) represents the dimerization motif. -
Post-translational
modificationsPhosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells. - Information by UniProt
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Database links
- Entrez Gene: 55143 Human
- Omim: 609977 Human
- SwissProt: Q53HL2 Human
- Unigene: 524571 Human
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Alternative names
- BOR antibody
- BOREA_HUMAN antibody
- Borealin antibody
see all
Images
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All lanes : Anti-Borealin/CDCA8 antibody (ab67126) at 1/500 dilution
Lane 1 : Borealin/CDCA8 transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Secondary
All lanes : Goat anti mouse IgG (H&L)-HRP conjugate secondary
antibody at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab67126 has been referenced in 2 publications.
- Peng R et al. Identification of Core Genes Involved in the Metastasis of Clear Cell Renal Cell Carcinoma. Cancer Manag Res 12:13437-13449 (2020). PubMed: 33408516
- Pike T et al. PKC? switches Aurora B specificity to exit the abscission checkpoint. Nat Commun 7:13853 (2016). WB, ICC/IF . PubMed: 28004745