Anti-Indoleamine 2, 3-dioxygenase antibody (ab106134)

My name is Jeremy Kasanovand I'm helping Sybille with her emails while she is out of the office.

The antibody was tested by thelab which developed this antibodyfor cross reactivity with mouse IDO in Western blot. Unfortunately we have no details which control material was used.

As for the reactivity on rat IDO, the details as confirmed in reference is the only data available.
Sorry I can't be more helpful in this instance. Please contact us again if you have any further questions.

Thank you for your reply.

The information the lab sent me is all we have available. There is also a reference listed on the datasheet where human and rat tissue was used:

Hill M et al. Heme oxygenase-1 inhibits rat and human breast cancer cell proliferation: mutual cross inhibition with indoleamine 2,3-dioxygenase. FASEB J 19:1957-68 (2005). Human, Rat. PubMed: 16319139

I will check again regarding mouse testing.

Also, I checkedthe human protein Atlas entry for IDO1 (http://www.proteinatlas.org/ENSG00000131203) where you will find more information about normal and cancer tissues as well as cell lines that express the protein and to which extend. For instance human lymphnodes express the protein strongly (http://www.proteinatlas.org/ENSG00000131203/normal). Human ovarian and cervical cancer tissue expresses the protein as well (http://www.proteinatlas.org/ENSG00000131203/cancer).

We have a collection of tissue slides that you can use as positive control slides - mainly human (https://www.abcam.com/index.html?c=2300), some for mouse (https://www.abcam.com/index.html?c=2301) and also a few for rat.

We do carry human lymph node slides (ab4350, https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

I am very pleased to hear you would like to use ab106134 in mouse and send us an image of your results.

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview with the image formouse and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you very much for your interest in ab106134 and for your patience.

Unfortunately I have not been able to obtain any more information on the tissue tested in mouse and I do not want for you to have to continue to wait for a response.


Since we do not have an image available, I can offer a discount off a future purchase if you buy ab106134 now, use it in mouse and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.

It doesn't matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab106134 in mouse. I will then send a discount code. This code must be issued before purchasing ab103164 so please wait for my reply before ordering.

2. Purchase ab106134 either by phone, fax, or online (www.abcam.com).

3. Use it in mouse.

4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

I am very pleased to hear you would like to accept our offer and test ab55305 and ab106134 in monkey. These codes will give you 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for each antibody in monkey and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.