Overview

  • Product name

    Anti-Indoleamine 2, 3-dioxygenase antibody
    See all Indoleamine 2, 3-dioxygenase primary antibodies
  • Description

    Goat polyclonal to Indoleamine 2, 3-dioxygenase
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    CVLANWKKKDPNK

    , corresponding to internal sequence amino acids 129-141 of Human Indoleamine 2, 3-dioxygenase (NP_002155.1).

  • Positive control

    • Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab134197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml.

Approx 50kDa band observed in Human Lymph Node, Spleen and Placenta lysates (calculated MW of 45.3kDa according to NP_002155.1).

IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
  • Pathway

    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
  • Sequence similarities

    Belongs to the indoleamine 2,3-dioxygenase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-dioxygenase antibody
    • I23O1_HUMAN antibody
    • IDO 1 antibody
    • IDO antibody
    • IDO-1 antibody
    • IDO1 antibody
    • INDO antibody
    • indolamine 2,3 dioxygenase antibody
    • Indole 2 3 dioxygenase antibody
    • indoleamine 2 3 dioxygenase 1 antibody
    • indoleamine 2 3 dioxygenase antibody
    • Indoleamine 2,3-dioxygenase 1 antibody
    • Indoleamine pyrrole 2 3 dioxygenase antibody
    • Indoleamine-pyrrole 2 antibody
    see all

Images

  • All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody (ab134197) at 0.5 µg/ml

    Lane 1 : Human lymph node lysate
    Lane 2 : Spleen lysate
    Lane 3 : Placenta lysate

    Lysates/proteins at 35 µg per lane.
  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Indoleamine 2, 3-dioxygenase with ab134197 at 4 µg/ml dilution.

References

This product has been referenced in:

  • Zhou CC  et al. Hepatic NAD(+) deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing. Br J Pharmacol 173:2352-68 (2016). Read more (PubMed: 27174364) »
  • Strong MJ  et al. Differences in gastric carcinoma microenvironment stratify according to EBV infection intensity: implications for possible immune adjuvant therapy. PLoS Pathog 9:e1003341 (2013). IHC-P ; Human . Read more (PubMed: 23671415) »
See all 2 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you for your email.

Since you have placed the order already, you would apply the discount code on the next order. I don't think you can retroactively add the discount code now as the order has already been processed, and the item was delivered today.


Yes, let me know if the results with ab134197 improve with another antigen retrieval method.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (colon)
Specification
colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
Yes - 0.3%Trix 100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mei Deng

Verified customer

Submitted Aug 23 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (colon)
Specification
colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate Buffer, pH6.0
Permeabilization
Yes - 0.3%Trix 100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mei Deng

Verified customer

Submitted Aug 23 2012

Answer

Thank you for your reply.

Since you have submitted the Abreview for ab134197, you can now use the activated discount code on a future order. Thus, if you would like to receive ab106134, you would place an order and apply the discount code. You can also call us (888-772-2226) to have the discount code added to your order or give it to your purchasing agentto do so.

Yes, in the future, please let us know if you like to try an antibody or other product in an untested application or species, so thatyou can take advantage of our testing discount program. We'd be happy to help you.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your email.

I had sent a reply to you 5 minutes before I received your second email. Our emails must have crossed each other on the way.

For ab134197, please see my previous email.

For ab106134, this antibody would be guaranteed for mouse and rat samples as well as IHC-P. The complaint you saw, could be from the same customer. Nevertheless, the antibody would be guaranteed. Sometimes a vial or a lot might not be working for a customer and the cause is not always easy to decipher.

As for ab27924 and ab55305, they both have been tested with human samples in IHC-P. Mouse and rat samples have not yet been tested. Thus, you could use our testing discount program (see my previous email for the details) for trying the antibodies in mouse and rat.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your email.

I am glad to hear the antibody ab134197 works well with human samples - the species the antibody has been tested with. However, I am sorry to hear that it does not seem to work in mouse and rat. We have not tested these species so far, but they were predicted to work based on sequence homology.

I would suggest trying a different antigen retrieval method with the mouse and rat samples (e.g. Tris/EDTA or enzymatic). Other than that your protocol looks ine and it worked well with human samples.


For trying an antibody in an untested species, we give out testing discount codes. I can retroactively send you the code as well as information about how this works:

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.


DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab134197 in mouse and rat. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse and/or ratand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab106134.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question
Answer

DISCOUNT CODE: ***
Expiration date: November 11, 2012

I am very pleased to hear you would like to accept our offer and test ab134197with rat samples.This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab134197 with rat samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

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