Anti-Indoleamine 2, 3-dioxygenase antibody (ab134197)
Key features and details
- Goat polyclonal to Indoleamine 2, 3-dioxygenase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Indoleamine 2, 3-dioxygenase antibody
See all Indoleamine 2, 3-dioxygenase primary antibodies -
Description
Goat polyclonal to Indoleamine 2, 3-dioxygenase -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog -
Immunogen
Synthetic peptide:
CVLANWKKKDPNK
, corresponding to internal sequence amino acids 129-141 of Human Indoleamine 2, 3-dioxygenase (NP_002155.1). -
Positive control
- Human colon tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab134197 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.5 - 1.5 µg/ml.
A 1 hour primary incubation is recommended for this product. Approx 50kDa band observed in Human Lymph Node, Spleen and Placenta lysates (calculated MW of 45.3kDa according to NP_002155.1). |
IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.5 - 1.5 µg/ml. A 1 hour primary incubation is recommended for this product. Approx 50kDa band observed in Human Lymph Node, Spleen and Placenta lysates (calculated MW of 45.3kDa according to NP_002155.1). |
IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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Database links
- Entrez Gene: 3620 Human
- Entrez Gene: 15930 Mouse
- Entrez Gene: 66029 Rat
- Omim: 147435 Human
- SwissProt: P14902 Human
- SwissProt: P28776 Mouse
- SwissProt: Q9ERD9 Rat
- Unigene: 840 Human
see all -
Alternative names
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Indoleamine 2, 3-dioxygenase with ab134197 at 6 µg/ml dilution. Heat induced antigen retrieval with citrate buffer pH 6.
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Negative control: Immunohistochemical analysis of paraffin-embedded Human placenta tissue with no primary antibody.
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All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody (ab134197) at 0.5 µg/ml
Lane 1 : Human lymph node lysate
Lane 2 : Spleen lysate
Lane 3 : Placenta lysate
Lysates/proteins at 35 µg per lane.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab134197 has been referenced in 5 publications.
- Ikeda N et al. Detection of indoleamine 2,3-dioxygenase 1-expressing cells in canine normal and tumor tissues. J Vet Med Sci 83:1885-1890 (2021). PubMed: 34690223
- Meng Y et al. GBP1 Facilitates Indoleamine 2,3-Dioxygenase Extracellular Secretion to Promote the Malignant Progression of Lung Cancer. Front Immunol 11:622467 (2020). PubMed: 33552086
- Mitchell SJ et al. Nicotinamide Improves Aspects of Healthspan, but Not Lifespan, in Mice. Cell Metab 27:667-676.e4 (2018). PubMed: 29514072
- Zhou CC et al. Hepatic NAD(+) deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing. Br J Pharmacol 173:2352-68 (2016). PubMed: 27174364
- Strong MJ et al. Differences in gastric carcinoma microenvironment stratify according to EBV infection intensity: implications for possible immune adjuvant therapy. PLoS Pathog 9:e1003341 (2013). IHC-P ; Human . PubMed: 23671415