Overview

  • Product name

    Anti-Indoleamine 2, 3-dioxygenase antibody
    See all Indoleamine 2, 3-dioxygenase primary antibodies
  • Description

    Rabbit polyclonal to Indoleamine 2, 3-dioxygenase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Indoleamine 2, 3-dioxygenase aa 1-403.
    Sequence:

    MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLI ESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVR KVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDV LFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKAL LEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMP PAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIV TKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLL KEG


    Database link: P14902

  • Positive control

    • WB: HeLa, K562, A549, HEK-293T and U-87 MG whole cell lysates. IHC-P: Human cervical cancer tissue. ICC/IF: HepG2 and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab235902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 45 kDa.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
  • Pathway

    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
  • Sequence similarities

    Belongs to the indoleamine 2,3-dioxygenase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-dioxygenase antibody
    • I23O1_HUMAN antibody
    • IDO 1 antibody
    • IDO antibody
    • IDO-1 antibody
    • IDO1 antibody
    • INDO antibody
    • indolamine 2,3 dioxygenase antibody
    • Indole 2 3 dioxygenase antibody
    • indoleamine 2 3 dioxygenase 1 antibody
    • indoleamine 2 3 dioxygenase antibody
    • Indoleamine 2,3-dioxygenase 1 antibody
    • Indoleamine pyrrole 2 3 dioxygenase antibody
    • Indoleamine-pyrrole 2 antibody
    see all

Images

  • All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody (ab235902) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 45 kDa

  • Paraffin-embedded human cervical cancer tissue stained for Indoleamine 2, 3-dioxygenase using ab235902 at 1/100 dilution in immunohistochemical analysis.

  • HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Indoleamine 2, 3-dioxygenase (green) using ab235902 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Anti-Indoleamine 2, 3-dioxygenase antibody (ab235902) at 1/500 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 45 kDa

  • 4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Indoleamine 2, 3-dioxygenase (green) using ab235902 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

References

ab235902 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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