Product nameAnti-Indoleamine 2, 3-dioxygenase antibody [BLR040F] - BSA free
See all Indoleamine 2, 3-dioxygenase primary antibodies
DescriptionRabbit monoclonal [BLR040F] to Indoleamine 2, 3-dioxygenase - BSA free
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Indoleamine 2, 3-dioxygenase.
Database link: P14902
This product is sold under License from Bethyl Laboratories, Inc.
ab272090 is the carrier-free version of ab243896. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab272090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
FunctionCatalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
PathwayAmino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Sequence similaritiesBelongs to the indoleamine 2,3-dioxygenase family.
- Information by UniProt
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
Indoleamine 2,3-Dioxygenase was immunoprecipitated from 1.0 mg HeLa whole cell lysate with ab243896 at 15 µl per reaction. Western blot was performed on the immunoprecipitate using ab243896 at 1/1000 dilution.
Lane 1: ab243896 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243896).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272090 has not yet been referenced specifically in any publications.