Product nameAnti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y]
See all Indoleamine 2, 3-dioxygenase primary antibodies
DescriptionRabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase
Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
A synthetic peptide corresponding to residues within human Indoleamine 2, 3-dioxygenase
- WB: HeLa cell lysate treated with IFN gamma. ICC/IF: HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab76157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/2500 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
FunctionCatalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
PathwayAmino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Sequence similaritiesBelongs to the indoleamine 2,3-dioxygenase family.
- Information by UniProt
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
ab76157 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76157 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/5000 dilution + HeLa cell lysate treated with IFN gamma at 10 µg
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
This product has been referenced in:
- Takeshita K et al. Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration. Stem Cell Res Ther 8:101 (2017). WB, ICC/IF ; Human . Read more (PubMed: 28446226) »
- Yamazaki T et al. Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in human epithelial HeLa cells treated with interferon-?. J Infect Chemother 20:460-4 (2014). Human . Read more (PubMed: 24855914) »