Recombinant
RabMAb

Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)

Overview

  • Product name
    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y]
    See all Indoleamine 2, 3-dioxygenase primary antibodies
  • Description
    Rabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues within human Indoleamine 2, 3-dioxygenase

  • Positive control
    • WB: HeLa cell lysate treated with IFN gamma. ICC/IF: HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/2500 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
    • Pathway
      Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
    • Sequence similarities
      Belongs to the indoleamine 2,3-dioxygenase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3-dioxygenase antibody
      • I23O1_HUMAN antibody
      • IDO 1 antibody
      • IDO antibody
      • IDO-1 antibody
      • IDO1 antibody
      • INDO antibody
      • indolamine 2,3 dioxygenase antibody
      • Indole 2 3 dioxygenase antibody
      • indoleamine 2 3 dioxygenase 1 antibody
      • indoleamine 2 3 dioxygenase antibody
      • Indoleamine 2,3-dioxygenase 1 antibody
      • Indoleamine pyrrole 2 3 dioxygenase antibody
      • Indoleamine-pyrrole 2 antibody
      see all

    Images

    • ab76157 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76157 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/5000 dilution + HeLa cell lysate treated with IFN gamma at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    References

    This product has been referenced in:
    • Takeshita K  et al. Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration. Stem Cell Res Ther 8:101 (2017). WB, ICC/IF ; Human . Read more (PubMed: 28446226) »
    • Yamazaki T  et al. Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in human epithelial HeLa cells treated with interferon-?. J Infect Chemother 20:460-4 (2014). Human . Read more (PubMed: 24855914) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    60 µg
    Gel Running Conditions
    Non-reduced Denaturing (gel 10 %)
    Sample
    Dog Cell lysate - whole cell (adipose tissue derived mesencymal stem cell)
    Specification
    adipose tissue derived mesencymal stem cell
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

    Dr. Seunghoon Lee

    Verified customer

    Submitted Sep 12 2013

    Following up my previous message, I received confirmation that, for the IDO antibody ab76157,  no testing has been done on rat samples. I can send this as a replacement but if you would rather have a credit or refund for ab106134, I can issue that inst...

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    The producer of this clone has provided the following information about the immunogen for ab76157: "Sequence homology of the immunogen with rat IDO is 86%. Species cross-reactivity data seems to be missing, so I will update you soon." It is not c...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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