Recombinant Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24032-22] to Indoleamine 2, 3-dioxygenase
- Suitable for: WB, IP
- Reacts with: Mouse
Overview
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Product name
Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22]
See all Indoleamine 2, 3-dioxygenase primary antibodies -
Description
Rabbit monoclonal [EPR24032-22] to Indoleamine 2, 3-dioxygenase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse Indoleamine 2, 3-dioxygenase aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P28776 -
Positive control
- WB: C2C12 (treated with 50ng/ml IFN-gamma (mouse) for 24 hours) whole cell lysate; Mouse colon tissue lysate. IP: Mouse colon tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24032-22 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab277522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa). | |
| IP | 1/30. |
Target
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Function
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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Database links
- Entrez Gene: 15930 Mouse
- SwissProt: P28776 Mouse
- Unigene: 392 Mouse
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Alternative names
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
see all
Images
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![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)](https://www.abcam.com/ps/products/277/ab277522/Images/ab277522-423583-anti-indoleamine-23dioxygenase-antibody-epr2403222-western-blot-myoblast.jpg)
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522) at 1/1000 dilution
Lane 1 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 2 : C2C12 treated with 50ng/ml IFN-gamma (mouse) for 24 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 30809284; PMID: 19741271).
Exposure time: 26 seconds.
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![Immunoprecipitation - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)](https://www.abcam.com/ps/products/277/ab277522/Images/ab277522-1-anti-indoleamine-23dioxygenase-antibody-epr2403222-immunoprecipitation-mouse-colon-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)Indoleamine 2,3-dioxygenase was immunoprecipitated from 0.35 mg mouse colon tissue lysate with ab277522 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277522 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse colon tissue lysate 10µg
Lane 2: ab277522 IP in mouse colon tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab277522 in mouse colon tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)](https://www.abcam.com/ps/products/277/ab277522/Images/ab277522-423584-anti-indoleamine-23dioxygenase-antibody-epr2403222-western-blot-lysate.jpg)
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522) at 1/1000 dilution + Mouse colon tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 19741271).
Exposure time: 5.5 seconds.
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![Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)](https://www.abcam.com/ps/products/277/ab277522/Images/ab277522-447368-benefitsofrecombinantantibodies.png)
Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab277522 has not yet been referenced specifically in any publications.
