Recombinant Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)](https://www.abcam.com/ps/products/277/ab277522/Images/ab277522-423583-anti-indoleamine-23dioxygenase-antibody-epr2403222-western-blot-myoblast.jpg "Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24032-22] to Indoleamine 2, 3-dioxygenase
- Suitable for: WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22]
See all Indoleamine 2, 3-dioxygenase primary antibodies -
Description
Rabbit monoclonal [EPR24032-22] to Indoleamine 2, 3-dioxygenase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C2C12 (treated with 50ng/ml IFN-gamma (mouse) for 24 hours) whole cell lysate; Mouse colon tissue lysate. IP: Mouse colon tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24032-22 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab277522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa). |
|
IP
1/30. |
Target
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Function
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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Database links
- Entrez Gene: 15930 Mouse
- SwissProt: P28776 Mouse
- Unigene: 392 Mouse
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Alternative names
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
see all
Images
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Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522) at 1/1000 dilution
Lane 1 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 2 : C2C12 treated with 50ng/ml IFN-gamma (mouse) for 24 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 30809284; PMID: 19741271).
Exposure time: 26 seconds.
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![Immunoprecipitation - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)](https://www.abcam.com/ps/products/277/ab277522/Images/ab277522-1-anti-indoleamine-23dioxygenase-antibody-epr2403222-immunoprecipitation-mouse-colon-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)Indoleamine 2,3-dioxygenase was immunoprecipitated from 0.35 mg mouse colon tissue lysate with ab277522 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277522 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse colon tissue lysate 10µg
Lane 2: ab277522 IP in mouse colon tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab277522 in mouse colon tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)](https://www.abcam.com/ps/products/277/ab277522/Images/ab277522-423584-anti-indoleamine-23dioxygenase-antibody-epr2403222-western-blot-lysate.jpg)
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522) at 1/1000 dilution + Mouse colon tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 19741271).
Exposure time: 5.5 seconds.
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![Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)](https://www.abcam.com/ps/products/277/ab277522/Images/ab277522-447368-benefitsofrecombinantantibodies.png)
Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab277522 has not yet been referenced specifically in any publications.
