Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-469669-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-western-blot-wildtype-a549-vehicle-control-ifn-gamma-0-ngml-48-h-wildtype-a549-tre.jpg "Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)")
Key features and details
- Mouse monoclonal [OTI1A3] to Indoleamine 2, 3-dioxygenase
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human, African green monkey
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3]
See all Indoleamine 2, 3-dioxygenase primary antibodies -
Description
Mouse monoclonal [OTI1A3] to Indoleamine 2, 3-dioxygenase -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human Indoleamine 2, 3-dioxygenase aa 1-403.
Sequence:MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLI ESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVR KVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDV LFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKAL LEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMP PAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIV TKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLL KEG
Database link: NP_002155 -
Positive control
- WB: Wild-type A549 Treated IFN gamma, SK-OV-3, HEK293T cell lysate transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase cDNA; IHC-P: Human kidney carcinoma, lymph node and lymphoma tissues; ICC/IF: COS7 cells transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI1A3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/4000. Predicted molecular weight: 45 kDa.
|
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| IHC-P | (1) |
1/150.
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| ICC/IF |
1/100.
|
| Notes |
|---|
|
WB
1/4000. Predicted molecular weight: 45 kDa. |
|
IHC-P
1/150. |
|
ICC/IF
1/100. |
Target
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Function
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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Database links
- Entrez Gene: 3620 Human
- Omim: 147435 Human
- SwissProt: P14902 Human
- Unigene: 840 Human
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Alternative names
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
see all
Images
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Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787) at 1/4000 dilution
Lane 1 : Wild-type A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate
Lane 2 : Wild-type A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate
Lane 3 : IDO1 knockout A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate
Lane 4 : IDO1 knockout A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate
Lane 5 : SK-OV-3 cell lysate
Lane 6 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lanes 1 - 6: Merged signal (red and green). Green - ab156787 observed at 40 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab156787 was shown to react with Indoleamine 2, 3-dioxygenase in treated wild-type A549 cells in Western blot with no signal observed in treated IDO1 knockout cell line ab266949 (IDO1 knockout cell lysate ab256948). Wild-type A549 and IDO1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab156787 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at a 1 in 4000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-163948-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution. -
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-163950-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-western-blot.jpg)
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787) at 1/4000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 45 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-163949-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase, labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/100 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-163947-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)](https://www.abcam.com/ps/products/156/ab156787/Images/ab156787-163946-anti-indoleamine-2-3-dioxygenase-antibody-oti1a3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787)Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab156787 has been referenced in 1 publication.
- Ye LY et al. Hypoxia-Induced Epithelial-to-Mesenchymal Transition in Hepatocellular Carcinoma Induces an Immunosuppressive Tumor Microenvironment to Promote Metastasis. Cancer Res 76:818-30 (2016). PubMed: 26837767
