Recombinant
RabMAb

Recombinant Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

Overview

  • Product name

    Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal
    See all Indoleamine 2, 3-dioxygenase primary antibodies
  • Description

    Rabbit monoclonal [SP260] to Indoleamine 2, 3-dioxygenase - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Indoleamine 2, 3-dioxygenase aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P14902

  • Positive control

    • Flow Cytometry: HeLa cells treated with IFN-gamma. IHC-P: Human tonsil, thymus, colon, stomach adenocarcinoma, pancreatic adenocarcinoma, endometrial adenocarcinoma and ovarian adenocarcinoma tissue. ICC/IF: HeLa cells
  • General notes

Properties

Applications

Our Abpromise guarantee covers the use of ab228468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/400.

Incubate for 30 minutes at 4°C.

IHC-P 1/100.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate for 10 minutes at room temperature.

 

ICC/IF 1/15.

Target

  • Function

    Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
  • Pathway

    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
  • Sequence similarities

    Belongs to the indoleamine 2,3-dioxygenase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-dioxygenase antibody
    • I23O1_HUMAN antibody
    • IDO 1 antibody
    • IDO antibody
    • IDO-1 antibody
    • IDO1 antibody
    • INDO antibody
    • indolamine 2,3 dioxygenase antibody
    • Indole 2 3 dioxygenase antibody
    • indoleamine 2 3 dioxygenase 1 antibody
    • indoleamine 2 3 dioxygenase antibody
    • Indoleamine 2,3-dioxygenase 1 antibody
    • Indoleamine pyrrole 2 3 dioxygenase antibody
    • Indoleamine-pyrrole 2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) treated with 50 ng/ml IFN-gamma for 16 h cells labeling Indoleamine 2, 3-dioxygenase with purified ab228468 at 1/15 (9.4µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow cytometry analysis of HeLa cells treated with 50ng/ml IFN-gamma for 16hours labeling Indoleamine 2, 3-dioxygenase with purified ab228468 at 1/200 dilution (0.705 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.Untreated cells (Green).

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry analysis of IFN-gamma treated HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling Indoleamine 2, 3-dioxygenase with ab228468 1/400 dilution (green) compared to a negative control rabbit IgG (blue).

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

References

This product has been referenced in:

  • Parra ER  et al. Immunohistochemical and Image Analysis-based Study Demonstrate that Several Immune Checkpoints are Co-expressed in Non-Small Cell Lung Carcinoma Tumors. J Thorac Oncol N/A:N/A (2018). Read more (PubMed: 29526824) »
  • Johnson DB  et al. Quantitative Spatial Profiling of PD-1/PD-L1 Interaction and HLA-DR/IDO-1 Predicts Improved Outcomes of Anti-PD-1 Therapies in Metastatic Melanoma. Clin Cancer Res 24:5250-5260 (2018). IHC-P . Read more (PubMed: 30021908) »
See all 2 Publications for this product

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