Recombinant Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)](https://www.abcam.com/ps/products/228/ab228470/Images/ab228470-293282-anti-indoleamine-2-3-dioxygenase-antibody-sp277-n-terminal-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP277] to Indoleamine 2, 3-dioxygenase - N-terminal
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal
See all Indoleamine 2, 3-dioxygenase primary antibodies -
Description
Rabbit monoclonal [SP277] to Indoleamine 2, 3-dioxygenase - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, lung, pancreatic adenocarcinoma, ovarian adenocarcinoma, B-cell lymphoma and endometrial adenocarcinoma tissue. HeLa cells treated with IFN.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP277 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary incubation for 10 minutes at room temperature. |
| Notes |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature. |
Target
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Function
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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Database links
- Entrez Gene: 3620 Human
- Omim: 147435 Human
- SwissProt: P14902 Human
- Unigene: 840 Human
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Alternative names
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)](https://www.abcam.com/ps/products/228/ab228470/Images/ab228470-293281-anti-indoleamine-2-3-dioxygenase-antibody-sp277-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)Formalin-fixed, paraffin-embedded human lung tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)](https://www.abcam.com/ps/products/228/ab228470/Images/ab228470-293280-anti-indoleamine-2-3-dioxygenase-antibody-sp277-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)](https://www.abcam.com/ps/products/228/ab228470/Images/ab228470-293278-anti-indoleamine-2-3-dioxygenase-antibody-sp277-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)](https://www.abcam.com/ps/products/228/ab228470/Images/ab228470-293277-anti-indoleamine-2-3-dioxygenase-antibody-sp277-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)](https://www.abcam.com/ps/products/228/ab228470/Images/ab228470-293276-anti-indoleamine-2-3-dioxygenase-antibody-sp277-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)](https://www.abcam.com/ps/products/228/ab228470/Images/ab228470-293275-anti-indoleamine-2-3-dioxygenase-antibody-sp277-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)Formalin-fixed, paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) treated with IFN stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)](https://www.abcam.com/ps/products/228/ab228470/Images/ab228470-293274-anti-indoleamine-2-3-dioxygenase-antibody-sp277-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)Formalin-fixed, paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.
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![Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)](https://www.abcam.com/ps/products/228/ab228470/Images/ab228470-9-benefits-of-recombinant-antibodies.png)
Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab228470 has not yet been referenced specifically in any publications.
