RecombinantRabMAb

Recombinant Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
  • Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP277] to Indoleamine 2, 3-dioxygenase - N-terminal
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal
    See all Indoleamine 2, 3-dioxygenase primary antibodies

  • Description

    Rabbit monoclonal [SP277] to Indoleamine 2, 3-dioxygenase - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil, lung, pancreatic adenocarcinoma, ovarian adenocarcinoma, B-cell lymphoma and endometrial adenocarcinoma tissue. HeLa cells treated with IFN.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab228470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
IHC-P
Human
WB
Mouse
Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

Target

  • Function

    Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.

  • Pathway

    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.

  • Sequence similarities

    Belongs to the indoleamine 2,3-dioxygenase family.
  • Information by UniProt

  • Database links

    • Alternative names

      • 3-dioxygenase antibody
      • I23O1_HUMAN antibody
      • IDO 1 antibody
      • IDO antibody
      • IDO-1 antibody
      • IDO1 antibody
      • INDO antibody
      • indolamine 2,3 dioxygenase antibody
      • Indole 2 3 dioxygenase antibody
      • indoleamine 2 3 dioxygenase 1 antibody
      • indoleamine 2 3 dioxygenase antibody
      • Indoleamine 2,3-dioxygenase 1 antibody
      • Indoleamine pyrrole 2 3 dioxygenase antibody
      • Indoleamine-pyrrole 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

      Formalin-fixed, paraffin-embedded human lung tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

      Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

      Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

      Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

      Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

      Formalin-fixed, paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) treated with IFN stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

      Formalin-fixed, paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

    • Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)
      Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal (ab228470)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab228470? Please let us know so that we can cite the reference in this datasheet.

    ab228470 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com