Recombinant
RabMAb

Recombinant Anti-Indoleamine 2, 3-dioxygenase antibody [SP282] (ab228472)

Overview

  • Product name

    Anti-Indoleamine 2, 3-dioxygenase antibody [SP282]
    See all Indoleamine 2, 3-dioxygenase primary antibodies
  • Description

    Rabbit monoclonal [SP282] to Indoleamine 2, 3-dioxygenase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Indoleamine 2, 3-dioxygenase aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: P14902

  • Positive control

    • IHC-P: Human tonsil, colon, colon adenocarcinoma, pancreatic adenocarcinoma, ovarian adenocarcinoma, endometrial adenocarcinoma, stomach adenocarcinoma and skin squamous cell carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab228472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
  • Pathway

    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
  • Sequence similarities

    Belongs to the indoleamine 2,3-dioxygenase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-dioxygenase antibody
    • I23O1_HUMAN antibody
    • IDO 1 antibody
    • IDO antibody
    • IDO-1 antibody
    • IDO1 antibody
    • INDO antibody
    • indolamine 2,3 dioxygenase antibody
    • Indole 2 3 dioxygenase antibody
    • indoleamine 2 3 dioxygenase 1 antibody
    • indoleamine 2 3 dioxygenase antibody
    • Indoleamine 2,3-dioxygenase 1 antibody
    • Indoleamine pyrrole 2 3 dioxygenase antibody
    • Indoleamine-pyrrole 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Indoleamine 2, 3-dioxygenase with ab228472 at 1/100 dilution (1.00 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228472 for 10 mins at room temperature.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Indoleamine 2, 3-dioxygenase using ab228472 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Indoleamine 2, 3-dioxygenase using ab228472 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase using ab228472 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase using ab228472 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase using ab228472 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase using ab228472 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase using ab228472 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Indoleamine 2, 3-dioxygenase using ab228472 at 1/100 dilution in immunohistochemical analysis.

References

ab228472 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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