Product nameIndoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit
See all Indoleamine 2, 3-dioxygenase kits
Sample typeCell culture extracts, Tissue Extracts
Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit (ab235936) enables IDO1 activity to be easily determined in mammalian tissues and cell lines. The assay uses a fluorogenic developer that selectively reacts with N-formylkynurenine (NFK) to produce a highly fluorescent product (Ex/Em = 402/88 nm), ensuring a high signal-to-background ratio. The kit also includes a highly selective IDO1 inhibitor for verification of enzyme activity in biological matrices. The assay has a simple no-wash protocol, is high-throughput adaptable and can detect down to 0.2 mU of IDO1 activity or 200 pmole NFK.
Indoleamine 2,3-Dioxygenase 1 (IDO1, EC 22.214.171.124) is a cytoplasmatic hemoprotein that oxidizes tryptophan yielding N-formylkynurenine (NFK). In mammals, this reaction is the first and rate limiting step in the kynurenine catabolic pathway. IDO1 activity is low under normal physiological conditions, but is dramatically upregulated by proinflammatory cytokines such as interferon-γ. This short-term activation of IDO1 occurs as part of the innate immune response and helps to inhibit the growth of pathogens and parasites. IDO1 activation can also promote host immuno tolerance by exerting an immunosuppressive effect. IDO1 expression by tumor cells plays a susbtancial role in tumor immune tolerance, aiding tumors in evading detection and destruction. Chronic induction of IDO1 expression has been found in cancer patients increased IDO1 activity is correlated with negative prognosis. IDO1 has thus become an attractice pharmacological target for development of novel antineoplastics and adjuvants to increase the efficacy of conventional chemotherapy.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests IDO1 Assay Buffer 1 x 50ml Antioxidant Mix (100X) 1 vial N-formylkynurenine Standard 1 vial IDO1 Substrate (L-tryptophan) 1 vial IDO1 Inhibitor (IDO5L) 1 vial Fluorogenic Developer Solution 1 x 5ml Recombinant Human IDO1 1 vial Microplate Sealing Film 1 unit
FunctionCatalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
PathwayAmino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Sequence similaritiesBelongs to the indoleamine 2,3-dioxygenase family.
- Information by UniProt
ab235936 has not yet been referenced specifically in any publications.