Key features and details
- Rabbit polyclonal to INF2 - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-INF2 antibody - N-terminal
See all INF2 primary antibodies
DescriptionRabbit polyclonal to INF2 - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment within Human INF2 (N terminal). The exact sequence is proprietary.
Database link: Q27J81
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.
This product was previously labelled as C14orf151
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 136 kDa.|
|IP||1/100 - 1/500.|
FunctionSevers actin filaments and accelerates their polymerization and depolymerization.
Tissue specificityWidely expressed. In the kidney, expression is apparent in podocytes and some tubule cells.
Involvement in diseaseDefects in INF2 are the cause of focal segmental glomerulosclerosis type 5 (FSGS5) [MIM:613237]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
Sequence similaritiesBelongs to the formin homology family.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
Contains 1 WH2 domain.
DomainThe WH2 domain acts as the DAD (diaphanous autoregulatory) domain and binds to actin monomers.
Regulated by autoinhibition due to intramolecular GBD-DAD binding.
The severing activity is dependent on covalent attachment of the FH2 domain to the C-terminus.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
- C14orf151 antibody
- C14orf173 antibody
- CMTDIE antibody
All lanes : Anti-INF2 antibody - N-terminal (ab229479) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 136 kDa
INF2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg ab229479. Western blot was performed from the immunoprecipitate using ab229479.
Lane 1: HEK-293T whole cell extract (Input)
Lane 2: Control IgG IP in HEK-293T whole cell extract.
Lane 3: ab229479 IP in HEK-293T whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229479 has not yet been referenced specifically in any publications.