Overview

  • Product name

    Anti-INF2 antibody - N-terminal
    See all INF2 primary antibodies
  • Description

    Rabbit polyclonal to INF2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment within Human INF2 (N terminal). The exact sequence is proprietary.
    Database link: Q27J81

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.
  • General notes

     This product was previously labelled as C14orf151

     

Properties

Applications

Our Abpromise guarantee covers the use of ab229479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 136 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Severs actin filaments and accelerates their polymerization and depolymerization.
  • Tissue specificity

    Widely expressed. In the kidney, expression is apparent in podocytes and some tubule cells.
  • Involvement in disease

    Defects in INF2 are the cause of focal segmental glomerulosclerosis type 5 (FSGS5) [MIM:613237]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities

    Belongs to the formin homology family.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
    Contains 1 WH2 domain.
  • Domain

    The WH2 domain acts as the DAD (diaphanous autoregulatory) domain and binds to actin monomers.
    Regulated by autoinhibition due to intramolecular GBD-DAD binding.
    The severing activity is dependent on covalent attachment of the FH2 domain to the C-terminus.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • C14orf151 antibody
    • C14orf173 antibody
    • CMTDIE antibody
    • DKFZp762A0214 antibody
    • FLJ22056 antibody
    • FSGS5 antibody
    • HBEAG binding protein 2 binding protein C antibody
    • HBEBP2 binding protein C antibody
    • HBEBP2-binding protein C antibody
    • INF 2 antibody
    • inf2 antibody
    • INF2_HUMAN antibody
    • Inverted formin 2 antibody
    • Inverted formin FH2 and WH2 domain containing antibody
    • Inverted formin-2 antibody
    • MGC13251 antibody
    • pp9484 antibody
    see all

Images

  • All lanes : Anti-INF2 antibody - N-terminal (ab229479) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 136 kDa



    5% SDS-PAGE

  • INF2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg ab229479. Western blot was performed from the immunoprecipitate using ab229479.

    Lane 1: HEK-293T whole cell extract (Input)

    Lane 2: Control IgG IP in HEK-293T whole cell extract.

    Lane 3: ab229479 IP in HEK-293T whole cell extract.

References

ab229479 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229479.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up