Overview

  • Product name
    Anti-Influenza A Virus antibody
    See all Influenza A Virus primary antibodies
  • Description
    Goat polyclonal to Influenza A Virus
  • Host species
    Goat
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Conjugationmore details
  • Species reactivity
    Reacts with: Influenza A
  • Immunogen

    Influenza A virus. Strain: USSR (H1N1).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Purification notes
    >95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/300.

This product may be used in place of neat antiserum in almost any appropriate antibody-based technique.

Conjugation Use at an assay dependent concentration.

Target

  • Relevance
    Influenza A and B are the two types of influenza viruses that cause epidemic human disease. Influenza type C infections cause a mild respiratory illness and are not thought to cause epidemics. Influenza A viruses are further categorized into subtypes on the basis of two surface antigens: hemagglutinin (H) and neuraminidase (N). Strains are also described by geographic origin, strain number and year of isolation.
  • Cellular localization
    Nuclear
  • Database links

    Images

    • ab20841 staining Influenza A Virus in reconstructed 1918 H1N1 influenza infected Mouse lung tissue sections by Immunohistochemistry. Tissue was fixed with 10% neutral-buffered formalin and embedded in paraffin. Slides were deparaffinized, rehydrated in water, steam antigen retrieved in pH 6 citrate buffer, and blocked with 3% H2O2. Samples were incubated with primary antibody (1/500) for 1 hour at room temperature. Top - EUK-207 treated mouse, 8 days postinfection, Bottom uninfected mouse control. Brown staining indicates positive staining in cell's cytoplasm and/or nucleus. Cellular debris is also frequently positive.

    References

    This product has been referenced in:
    • Slaine PD  et al. Adaptive Mutations in Influenza A/California/07/2009 Enhance Polymerase Activity and Infectious Virion Production. Viruses 10:N/A (2018). Read more (PubMed: 29783694) »
    • Slaine PD  et al. Stress Granule-Inducing Eukaryotic Translation Initiation Factor 4A Inhibitors Block Influenza A Virus Replication. Viruses 9:N/A (2017). Read more (PubMed: 29258238) »
    See all 16 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Flow Cytometry
    Sample
    Mouse Cell (mATII)
    Permeabilization
    No
    Gating Strategy
    CD45- CD31- MHCII+ EpCam+
    Specification
    mATII
    Preparation
    Cell harvesting/tissue preparation method: dispase/DNase digestion
    Sample buffer: 5% FBS DMEM

    Abcam user community

    Verified customer

    Submitted Jun 13 2018

    Answer

    Thank you for contacting Abcam regarding ab20841.


    I have confirmed that this antibody is against all proteinsin H1N1 influenza A virus and the alternative name was added in error. The antibody does not specifically target the M1 protein. The datasheet has been updated accordingly.


    I hope this information is helpful. Please do not hesitate to contact us if you have any additonal questions.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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