Key features and details
- Goat polyclonal to Influenza A Virus
- Suitable for: ICC/IF, IHC-P
- Reacts with: Influenza A
- Isotype: IgG
Product nameAnti-Influenza A Virus antibody
See all Influenza A Virus primary antibodies
DescriptionGoat polyclonal to Influenza A Virus
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Influenza A
Influenza A virus. Strain: USSR (H1N1).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityIon Exchange Chromatography
Purification notes>95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
Our Abpromise guarantee covers the use of ab20841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
This product may be used in place of neat antiserum in almost any appropriate antibody-based technique.
RelevanceInfluenza A and B are the two types of influenza viruses that cause epidemic human disease. Influenza type C infections cause a mild respiratory illness and are not thought to cause epidemics. Influenza A viruses are further categorized into subtypes on the basis of two surface antigens: hemagglutinin (H) and neuraminidase (N). Strains are also described by geographic origin, strain number and year of isolation.
ab20841 staining Influenza A Virus in reconstructed 1918 H1N1 influenza infected Mouse lung tissue sections by Immunohistochemistry. Tissue was fixed with 10% neutral-buffered formalin and embedded in paraffin. Slides were deparaffinized, rehydrated in water, steam antigen retrieved in pH 6 citrate buffer, and blocked with 3% H2O2. Samples were incubated with primary antibody (1/500) for 1 hour at room temperature. Top - EUK-207 treated mouse, 8 days postinfection, Bottom uninfected mouse control. Brown staining indicates positive staining in cell's cytoplasm and/or nucleus. Cellular debris is also frequently positive.
ab20841 has been referenced in 17 publications.
- Slaine PD et al. Adaptive Mutations in Influenza A/California/07/2009 Enhance Polymerase Activity and Infectious Virion Production. Viruses 10:N/A (2018). PubMed: 29783694
- Slaine PD et al. Stress Granule-Inducing Eukaryotic Translation Initiation Factor 4A Inhibitors Block Influenza A Virus Replication. Viruses 9:N/A (2017). PubMed: 29258238
- Silver JS et al. Inflammatory triggers associated with exacerbations of COPD orchestrate plasticity of group 2 innate lymphoid cells in the lungs. Nat Immunol 17:626-35 (2016). PubMed: 27111143
- Khaperskyy DA et al. Selective Degradation of Host RNA Polymerase II Transcripts by Influenza A Virus PA-X Host Shutoff Protein. PLoS Pathog 12:e1005427 (2016). WB, IF . PubMed: 26849127
- Peteranderl C et al. Macrophage-epithelial paracrine crosstalk inhibits lung edema clearance during influenza infection. J Clin Invest 126:1566-80 (2016). PubMed: 26999599