Key features and details
- Mouse monoclonal [2B7] to Influenza A virus (H5N1/HA1)
- Suitable for: WB, ELISA
- Reacts with: Influenza A
- Isotype: IgG1
Product nameAnti-Influenza A virus (H5N1/HA1) antibody [2B7]
DescriptionMouse monoclonal [2B7] to Influenza A virus (H5N1/HA1)
Specificityab135382 specifically recognizes H5 (H5N1/HA1) recombinant protein, but not interacted H1N1/HA1 and H3N2/HA1 recombinant protein. This antibody has been tested with the following: H5N1/HA (Influenza A virus (A/Vietnam/HN31242/2007H5N1) haemagglutinin), H3N2 (Influenza A virus (A/Perth/16/2009(H3N2) segment 4 hemagglutinin (HA) gene), and H1N1/HA1 (Influenza A virus (A/New York/3571/2009 H1N1) haemagglutinin).
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Influenza A
Recombinant protein, purified from Baculovirus corresponding to amino acids 17-338 of Influenza A virus (H5N1/HA1)
- H5 (H5N1) recombinant protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab135382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/3000. Predicted molecular weight: 64 kDa.|
|ELISA||Use at an assay dependent dilution.|
All lanes : Anti-Influenza A virus (H5N1/HA1) antibody [2B7] (ab135382) at 1/3000 dilution
Lane 1 : H1N1 Recombinant protein
Lane 2 : H3N2 Recombinant protein
Lane 3 : H5N1 Recombinant protein
Lysates/proteins at 0.05 µg per lane.
All lanes : Goat Anti mouse HRP
Developed using the ECL technique.
Predicted band size: 64 kDa
ab135382 at 1 µg/ml specifically recognizes H5N1/HA1 recombinant protein, but not interacted H1N1/HA1 and H3N2/HA1 recombinant protein in ELISA.
ab135382 has been referenced in 4 publications.
- Wang G et al. The G Protein-Coupled Receptor FFAR2 Promotes Internalization during Influenza A Virus Entry. J Virol 94:N/A (2020). PubMed: 31694949
- Das DK et al. Direct Visualization of the Conformational Dynamics of Single Influenza Hemagglutinin Trimers. Cell 174:926-937.e12 (2018). PubMed: 29961575
- Ahmed SR et al. In situ self-assembly of gold nanoparticles on hydrophilic and hydrophobic substrates for influenza virus-sensing platform. Sci Rep 7:44495 (2017). PubMed: 28290527
- Ahmed SR et al. Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus. Nanotheranostics 1:338-345 (2017). PubMed: 29071197