Overview

  • Product name
    Anti-Influenza A Virus Hemagglutinin antibody [C102]
    See all Influenza A Virus Hemagglutinin primary antibodies
  • Description
    Mouse monoclonal [C102] to Influenza A Virus Hemagglutinin
  • Host species
    Mouse
  • Specificity
    This antibody reacts with haemagglutinin of H1N1 serotype. We have tested the antibody with following strains: Strain - Result A/Beijing/262/95 - positive A/Leningrad/325/88 - negative A/New Caledonia/20/99 - positive A/Taiwan/1/86 - positive A/Kiev/301/94 - negative A/Panama/2007/99 - negative A/Shangdong/9/93 - negative A/Texas/1/77 - positive B/Leningrad/86/93 - negative B/Qingdao/102/91 - negative
  • Tested applications
    Suitable for: Inhibition Assay, ICC/IF, IHC-Fr, ELISAmore details
  • Immunogen

    BALB/c mice immunised with purified influenza virus type A strain H1N1 (strain A/Kazakhstan/Seagull/470).

  • General notes

    This antibody is directed against influenza virus type A, (haemagglutinin).

     

Properties

Applications

Our Abpromise guarantee covers the use of ab8262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration. Also can be used for serotyping of influenza virus type A and haemagglutinin inhibition.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Influenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong. There was some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species jumping ability. Influenza A Virus Hemagglutinin antibodies recognize the influenza hemagglutinin epitope, which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of this antibody allows for unambiguous identification and quantitative analysis of the tagged protein.
  • Cellular localization
    Apical cell membrane; Single-pass type I membrane protein. Note=Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts.
  • Alternative names
    • HA antibody
    • HA1 antibody
    • HA2 antibody
    • Hemagglutinin antibody

References

This product has been referenced in:
  • Liu CH  et al. Localization and force analysis at the single virus particle level using atomic force microscopy. Biochem Biophys Res Commun 417:109-15 (2012). Read more (PubMed: 22138243) »
  • Yu B  et al. A method to generate recombinant Salmonella typhi Ty21a strains expressing multiple heterologous genes using an improved recombineering strategy. Appl Microbiol Biotechnol 91:177-88 (2011). WB . Read more (PubMed: 21611798) »
See all 3 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Answer

Thank you for your inquiry.

I attached the Certificates of Complianceforthese antibodies asPDF.

I wish you and your customer a good weekend.

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Answer

Thank you for your enquiry.

Please find enclosed the requested CoC for these antibodies (ab8262, ab66187, ab66190) as attachments.

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for your enquiry and your interest.

We can provide the concentration of these products which are listed on the on-line datasheets. Currently, we can't provide the titer.

Let m know if you need the CoC without the titer data or not.

I look forward to hearing from you soon.

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Answer

Thank you for your enquiry. I would suggest your customer taking a look at the datasheet of ab8262, it is exactly the same clone C102 as the unpublished product (ab20999). I hope this helps and if I can assist further, please do not hesitate to contact

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Answer

Thank you for contacting us. Ab8262 is raised against whole purified influenza virus type A strain H1N1 (strain A/Kazakhstan/Seagull/470) and unfortunately as yet no precise epitope mapping has been performed. However, we do know that the antibody reacts with haemagglutinin of H1N1 serotype. I'm sorry I could not be of more help, but please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your inquiry. I am verry sorry to say that to our knowledge the epitope has not been determined. The monoclonal antibody clone C102 was obtained by use of avian influenza virus strain A (H1N1) as an immunogen and it is directed against relatively conservative H1 epitope. Unfortunately we do not have the sequence of the immunogen available. The antibodies cross-reactivity pattern shows that it does not react with H3 and other hemagglutinins but interacts with H1 from human and avian influenza viruses.

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Answer

Thank you for your enquiry. Please find the protocol for ab8262 and ab2053 included below. A basic ELISA protocol can be used with these antibodies. I am still looking into ab20999, and will contact you with more information when I receive it. ELISA .Coat antigen on plate in 0.1 M Carbonate buffer, pH 9.3, 0.1 ml/well. Incubate overnight at 40 C,. .Add MAb in PBSAT (PBS, 2 mg/ml BSA, 0.1% Tween 20), 0.1 ml/well. Incubate for 1 h at 37 0C. .Add Conjugate a-IgG(m)-HRP in PBSAT, 0.1 ml/well. Incubation 1 h at 370C .Add substrate TMB - 0.1 ml/well. Develop color 15 min with stirring at the room temperature. The reaction is stopped with 4.8% H2SO4 (100 ml/well) and OD450 was checked. Blank wells contained no Mabs. The wells were washed after each incubation with PBST (PBS, 0.05% Tween 20) - 0.15 ml/well, 5 times. We have tested the antibodies with following strains: Ab8262: Strain Result A/Beijing/262/95 positive A/Leningrad/325/88 negative A/New Caledonia/20/99 positive A/Taiwan/1/86 positive A/Kiev/301/94 negative A/Panama/2007/99 negative A/Shangdong/9/93 negative A/Texas/1/77 positive B/Leningrad/86/93 negative B/Qingdao/102/91 negative Ab2053: Strain Result A/New Caledonia/20/99 negative A/Beijing/262/95 negative A/Taiwan/1/86 negative A/Johannesburg/33/94 negative A/Kiev/301/94 negative A/Panama/2007/99 positive A/Shangdong/9/93 negative

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Answer

Thank you for your enquiry. Unfortunately the epitope for the antibody ab8262 has not been mapped, therefore we do not know which part of the protein is recognized by the antibody. Sorry I couldn't help you more on this occasion,

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Question
Answer

This antibody has been successfully tested for application in IHC-frozen sections, but unfortunately an image is not available at this time. Please contact us again if you have any additional questions.

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Answer

Thank you for your enquiry. ab8262 was raised using the immunogen influenza A virus (H1N1), strain A/Kazakhstan/Seagull/470. I do not have specific details about the cross reactivity of this antiserum. However, I can tell you that according to the Abreview submitted from this antibody recognizes the WSN strain of Influenza A Virus Hemagglutinin. We do not offer free or trial sized vials. However, I would be keen to see how this antibody performs using FACs and therefore like to offer you a 20% discount. If you are interested, e-mail me and I will supply you with a unique discount code which you can use when you place the order. In return I would ask you to submit an "Abreview" by navigating to the product web page at Abcam.com and clicking on the Abreview tab. I look forward to hearing from you.

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1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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