Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)
- Datasheet
- Specific References (33)
- Protocols
Overview
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Product nameAnti-Influenza A Virus M2 Protein antibody [14C2]
See all Influenza A Virus M2 Protein primary antibodies -
DescriptionMouse monoclonal [14C2] to Influenza A Virus M2 Protein
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Host speciesMouse
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Tested applicationsSuitable for: Inhibition Assay, Flow Cyt, ICC/IF, ICC, IP, WBmore details
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Immunogen
Tissue, cells or virus corresponding to Influenza A Virus M2 Protein. Full length M2 protein from A/WSN/33-infected CV1 cell lysate.
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EpitopeDetects the N-terminal of the Influenza A Virus M2 Protein.
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Positive control
- ICC/IF: Infected MDCK cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
PurityProtein G purified
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Primary antibody notesInfluenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
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ClonalityMonoclonal
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Clone number14C2
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab5416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Inhibition Assay | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use a concentration of 1 µg/ml. | |
| ICC | Use a concentration of 1 µg/ml. | |
| IP | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa. |
Target
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RelevanceThe Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
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Cellular localizationVirion membrane. Apical cell membrane; Single-pass type III membrane protein.
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Alternative names
- Influenza A virus matrix protein M2 antibody
- M antibody
- M2 antibody
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Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)](https://www.abcam.com/ps/datasheet/Images/5/ab5416/ab5416_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)Immunofluorescence staining of infected MDCK cells using ab5416.
Protocols
References
This product has been referenced in:
- Tutykhina I et al. Vaccination potential of B and T epitope-enriched NP and M2 against Influenza A viruses from different clades and hosts. PLoS One 13:e0191574 (2018). Read more (PubMed: 29377916) »
- Opriessnig T et al. Comparison of the efficacy of a commercial inactivated influenza A/H1N1/pdm09 virus (pH1N1) vaccine and two experimental M2e-based vaccines against pH1N1 challenge in the growing pig model. PLoS One 13:e0191739 (2018). WB . Read more (PubMed: 29381710) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"