Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)
![Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)](https://www.abcam.com/ps/datasheet/Images/5/ab5416/ab5416_1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)")
Key features and details
- Mouse monoclonal [14C2] to Influenza A Virus M2 Protein
- Suitable for: Inhibition Assay, Flow Cyt, ICC/IF, ICC, IP, WB
- Reacts with: Dog, Influenza A
- Isotype: IgG1
Overview
-
Product name
Anti-Influenza A Virus M2 Protein antibody [14C2]
See all Influenza A Virus M2 Protein primary antibodies -
Description
Mouse monoclonal [14C2] to Influenza A Virus M2 Protein -
Host species
Mouse -
Tested applications
Suitable for: Inhibition Assay, Flow Cyt, ICC/IF, ICC, IP, WBmore details -
Species reactivity
Reacts with: Dog, Influenza A -
Immunogen
Tissue, cells or virus corresponding to Influenza A Virus M2 Protein. Full length M2 protein from A/WSN/33-infected CV1 cell lysate.
-
Epitope
Detects the N-terminal of the Influenza A Virus M2 Protein. -
Positive control
- ICC/IF: Infected MDCK cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication. -
Clonality
Monoclonal -
Clone number
14C2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Inhibition Assay |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
|
| ICC | (1) |
Use a concentration of 1 µg/ml.
|
| IP |
Use a concentration of 10 µg/ml.
|
|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa.
|
| Notes |
|---|
|
Inhibition Assay
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
ICC
Use a concentration of 1 µg/ml. |
|
IP
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa. |
Target
-
Relevance
The Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment. -
Cellular localization
Virion membrane. Apical cell membrane; Single-pass type III membrane protein. -
Database links
- Entrez Gene: 1461002 Influenza A
- Entrez Gene: 956528 Influenza A
- SwissProt: P06821 Influenza A
- SwissProt: Q67179 Influenza A
-
Alternative names
- Influenza A virus matrix protein M2 antibody
- M antibody
- M2 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)Immunofluorescence staining of infected MDCK cells using ab5416.
Protocols
References (57)
ab5416 has been referenced in 57 publications.
- Blokhina EA et al. Plant-Produced Recombinant Influenza A Virus Candidate Vaccine Based on Flagellin Linked to Conservative Fragments of M2 Protein and Hemagglutintin. Plants (Basel) 9:N/A (2020). PubMed: 32013187
- Zhang X et al. Brevilin A, a Sesquiterpene Lactone, Inhibits the Replication of Influenza A Virus In Vitro and In Vivo. Viruses 11:N/A (2019). PubMed: 31500389
- Zahmanova GG et al. Rapid High-Yield Transient Expression of Swine Hepatitis E ORF2 Capsid Proteins in Nicotiana benthamiana Plants and Production of Chimeric Hepatitis E Virus-Like Particles Bearing the M2e Influenza Epitope. Plants (Basel) 9:N/A (2019). PubMed: 31878256
- Ma X et al. Transcription Factor EB Activation Rescues Advanced aB-Crystallin Mutation-Induced Cardiomyopathy by Normalizing Desmin Localization. J Am Heart Assoc 8:e010866 (2019). PubMed: 30773991
- Yao Y et al. Protection against homo and hetero-subtypic in?uenza A virus by optimized M2e DNA vaccine. Emerg Microbes Infect 8:45-54 (2019). PubMed: 30866759
