Overview

  • Product name
    Anti-Influenza A Virus M2 Protein antibody [14C2]
    See all Influenza A Virus M2 Protein primary antibodies
  • Description
    Mouse monoclonal [14C2] to Influenza A Virus M2 Protein
  • Host species
    Mouse
  • Tested applications
    Suitable for: Inhibition Assay, Flow Cyt, ICC/IF, ICC, IP, WBmore details
  • Species reactivity
    Reacts with: Influenza A
  • Immunogen

    Tissue, cells or virus corresponding to Influenza A Virus M2 Protein. Full length M2 protein from A/WSN/33-infected CV1 cell lysate.

  • Epitope
    Detects the N-terminal of the Influenza A Virus M2 Protein.
  • Positive control
    • ICC/IF: Infected MDCK cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
  • Clonality
    Monoclonal
  • Clone number
    14C2
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa.

Target

  • Relevance
    The Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
  • Cellular localization
    Virion membrane. Apical cell membrane; Single-pass type III membrane protein.
  • Database links
    • Alternative names
      • Influenza A virus matrix protein M2 antibody
      • M antibody
      • M2 antibody
      • Matrix protein 2 antibody
      • Membrane ion channel M2 antibody
      • Membrane protein M2 antibody
      • Proton channel protein M2 antibody
      see all

    Images

    • Immunofluorescence staining of infected MDCK cells using ab5416.

    References

    This product has been referenced in:
    • Tutykhina I  et al. Vaccination potential of B and T epitope-enriched NP and M2 against Influenza A viruses from different clades and hosts. PLoS One 13:e0191574 (2018). Read more (PubMed: 29377916) »
    • Opriessnig T  et al. Comparison of the efficacy of a commercial inactivated influenza A/H1N1/pdm09 virus (pH1N1) vaccine and two experimental M2e-based vaccines against pH1N1 challenge in the growing pig model. PLoS One 13:e0191739 (2018). WB . Read more (PubMed: 29381710) »
    See all 37 Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (influnza A infected)
    Specification
    influnza A infected
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C

    Miss. natalie blair

    Verified customer

    Submitted Jun 29 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (M2 transfected PG13's)
    Specification
    M2 transfected PG13's
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jun 16 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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