Key features and details
- Rabbit polyclonal to Influenza A Virus M2 Protein
- Suitable for: WB, ELISA
- Reacts with: Influenza A
- Isotype: IgG
Product nameAnti-Influenza A Virus M2 Protein antibody
See all Influenza A Virus M2 Protein primary antibodies
DescriptionRabbit polyclonal to Influenza A Virus M2 Protein
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Influenza A
A synthetic peptide corresponding to N terminal residues of Influenza A Virus M2 Protein
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab56086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
Cellular localizationVirion membrane. Apical cell membrane; Single-pass type III membrane protein.
- Influenza A virus matrix protein M2 antibody
- M antibody
- M2 antibody
ab56086 has been referenced in 5 publications.
- Sayhi M et al. Electrochemical detection of influenza virus H9N2 based on both immunomagnetic extraction and gold catalysis using an immobilization-free screen printed carbon microelectrode. Biosens Bioelectron 107:170-177 (2018). PubMed: 29455027
- Kawaguchi A et al. Influenza Virus Induces Cholesterol-Enriched Endocytic Recycling Compartments for Budozone Formation via Cell Cycle-Independent Centrosome Maturation. PLoS Pathog 11:e1005284 (2015). PubMed: 26575487
- Ebrahimi SM et al. Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2. Mol Biol Rep 37:2909-14 (2010). WB, ICC/IF ; Influenza A . PubMed: 19809890
- Ebrahimi SM & Tebianian M Heterologous expression, purification and characterization of the influenza A virus M2e gene fused to Mycobacterium tuberculosis HSP70(359-610) in prokaryotic system as a fusion protein. Mol Biol Rep 37:2877-83 (2010). ICC/IF . PubMed: 19813102
- Ebrahimi SM et al. Cloning, expression and purification of the influenza A (H9N2) virus M2e antigen and truncated Mycobacterium tuberculosis HSP70 as a fusion protein in Pichia pastoris. Protein Expr Purif : (2009). WB, ICC/IF . PubMed: 19897044