Overview

  • Product name

    Anti-Influenza A Virus M2 Protein antibody
    See all Influenza A Virus M2 Protein primary antibodies
  • Description

    Rabbit polyclonal to Influenza A Virus M2 Protein
  • Host species

    Rabbit
  • Specificity

    ab94925 detects Influenza A Virus M2 Protein. It may crossreact with M2 protein from other strains of influenza.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    A 13 amino acid peptide from the N terminus of Influenza A Virus M2 Protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity chromatography purified via peptide column.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 11 kDa.

Target

  • Relevance

    The Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
  • Cellular localization

    Virion membrane. Apical cell membrane; Single-pass type III membrane protein.
  • Alternative names

    • Influenza A virus matrix protein M2 antibody
    • M antibody
    • M2 antibody
    • Matrix protein 2 antibody
    • Membrane ion channel M2 antibody
    • Membrane protein M2 antibody
    • Proton channel protein M2 antibody
    see all

References

ab94925 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
ELISA
Sample
A/Sw/SK/2001 (H1N1) influenza Purified protein (Purified inactivated human H3N2 influenza virus)
Specification
Purified inactivated human H3N2 influenza virus
Type
Indirect

Abcam user community

Verified customer

Submitted Nov 02 2016

Answer

Thank you for your enquiry and interest in our products.
There is 92.31% homology between the immunogen used for ab94925 (Anti-Influenza A Virus M2 Protein antibody) and ACP44185 - EMBL: Matrix protein 2 - Influenza A virus (A/California/07/2009(H1N1).
If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for your enquiry.

The immunogens used to produce ab94925 and ab56086 consist of a small 15-20 amino acid peptide from Influenza A virus M2 protein. These peptides are not His tagged.

I hope this is helpful. Please contact me again if you have any further questions.

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Question
Answer

Following my colleague's email sent on Friday 23rd of December (CCE 3385949), we have been informed that there is 84% homology between the immunogen for ab94925 (www.abcam.com/ab94925) and the customer's sequence: [******* ****]. I hope this information is helpful.

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Answer

Thank you very much for your inquiry. I have contacted the laboratory for information on the ab5416. However the laboratory has not tested this antibody neither on your strain. I did a sequence alignement of the immunogen and your sequence. Please see the results below. Indeed, the alignement is very good. However as this is a monoclonal antibody, we do not know the epitope and can therefore not guarantee crossreactivity. http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111223-121219-0395-63524695-pg We do have two other antibodies against the Influenza A Virus M2 protein, the ab56086 and the ab94925. I have already done the alignement of the immunogen of ab56086 with your protein of interest. The overlapp is 100%. I would therefore expect that this antibody does recognize your protein as well. xxx I am checking with the laboratory for the alingment of ab94925 with your protein (http://www.uniprot.org/uniprot/A5GX42) and will get back to you. If the customer would like to test the ab56086 on the Jersey Strain, I would be pleased to provide also a testing discount (www.abcam.com/collaborationdiscount). Please let me know if you would like to know more about this testing discount. Please do not hesitate to contact me, should you or the customer have any other question or concern.

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