Overview

  • Product name
    Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC)
    See all Influenza A Virus Nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [431] to Influenza A Virus Nucleoprotein (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Specific for nucleoprotein of all Influenza A strains. The following isolates have been tested and found positive: A/Hong Kong/1/68 A/Leningrad/358/80 A/Sychuan/2/87 A/Zakarpatje/354/89 A/St. Petersburg/40/92 A/Beijing/32-92 A/Swine/1976/31 A/PR/8/34 A/FM/1/86 A/Leningrad/325/88 A/Texas/36/91 A/Taiwan/1/86 A/Leningrad/459/80 A/Hen/Kishinev/75 A/Duck/Alberta/35/76 A/Duck/Ukraine/1/63 A/Duck/California/72
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with the Influenza A virus.
  • Immunogen

    Purified virions.

  • General notes

    Conjugated with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab20921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/5 - 1/20. Acetone fixation of the antigen in a permissive tissue culture system is recommended prior to direct staining.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • Cellular localization
    Host cell nucleus
  • Alternative names
    • Common flu NP antibody
    • Influenza A virus NP antibody
    • NP antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Protein N antibody
    • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
    see all

Images

  • ab20921 staining Influenza A Virus Nucleoprotein in the Influenza infected Human B-cell lymphoma cell line by Flow Cytometry. Cells were cultured in PBS + 1% BSA and were fixed and permeabilized using a Kit. The sample was incubated with the primary antibody (1/50 in a wash) for 1 hour at 4°C.
    Gating Strategy: Single cell suspension.

References

This product has been referenced in:
  • Vashist N  et al. Influenza-Activated ILC1s Contribute to Antiviral Immunity Partially Influenced by Differential GITR Expression. Front Immunol 9:505 (2018). Mouse . Read more (PubMed: 29623077) »
  • Perot BP  et al. Autophagy diminishes the early interferon-ß response to influenza A virus resulting in differential expression of interferon-stimulated genes. Cell Death Dis 9:539 (2018). Read more (PubMed: 29748576) »
See all 17 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Gern geschehen, jederzeit gerne wieder!

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Answer

Vielen Dank für Ihren Anruf.

Wie versprochen habe ich im Labor wegen der Lagerbedingungen für den Anti-Influenza A Virus Nucleoprotein- Antikörper ab20921 nachgefragt, und folgendes wurde uns bestätigt:

Die Instruktion 'Upon receipt, aliquot and store at –20°C' besteht seit Februar 2006, die Lagerbedingungen davor besagten:'Short-term (up to 6 months) store at 2-8°C.Long term, aliquot and store at –20°C.' Das heißt, dass ab20921, der bis zu 6 Monate bei ca. +4°C gelagert war,getestet wurde und ohne Qualitätseinbuβenfunktioniert.Wie Sie vielleicht ganz richtig vermuten, reflektieren diese Hinweise allerdings eher die sich ändernden Garantiezeiträume und weniger die tatsächliche Haltbarkeit des Antikörpers.

Daher können wir Ihnen in diesem Fall für einen Zeitraum von 6 Monaten garantieren, dass ab20921 so funktioniert, wie auf dem Datenblatt angegeben, wenn Sie den Antikörper (wie bisher bei Ihnen validiert) bei +4°C lagern. Ich habe Ihnen dazu ein aktuelles Certificate of Compliance (CoC) für Ihre Unterlagen beigefügt.

Abcam übernimmt derzeit eine Garantie von sechs Monaten für alle Produkte (https://www.abcam.com/Abpromise). Bisher sind wir aber auch sehr flexibel und ersetzen bzw. schreiben fehlerhafte Antikörper im Einzelfall auch über einen Zeitraum von sechs Monaten hinaus gut.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Influenza infected B-cell lymphoma cell line)
Specification
Influenza infected B-cell lymphoma cell line
Preparation
Cell harvesting/tissue preparation method: Spin & Resuspend
Sample buffer: PBS w/ 1% BSA
Fixation
BD Fix/Perm Kit
Permeabilization
Yes - BD Fix/Perm Kit
Gating Strategy
Single cell suspension

Abcam user community

Verified customer

Submitted Oct 20 2011

Answer

Thank you for your enquiry. I am sincerely sorry to confirm that we do not have any data to provide regarding the crossreactivity of ab20921 Influenza A Virus Nucleoprotein antibody with Influenza A H1N1 Brisbane/10 or Brisbane 59. This has not been tested in the laboratory. I would be pleased to provide you with a 20% discount if you would like to purchase this antibody to test in these strains, and then provide us with some feedback and results by submitting an Abreview. Please let me know if you would like me to arrange this for you. To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. I hope this will be helpful. I look forward to hearing from you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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