Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC) (ab20921)


  • Product name
    Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC)
    See all Influenza A Virus Nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [431] to Influenza A Virus Nucleoprotein (FITC)
  • Host species
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Specific for nucleoprotein of all Influenza A strains. The following isolates have been tested and found positive: A/Hong Kong/1/68 A/Leningrad/358/80 A/Sychuan/2/87 A/Zakarpatje/354/89 A/St. Petersburg/40/92 A/Beijing/32-92 A/Swine/1976/31 A/PR/8/34 A/FM/1/86 A/Leningrad/325/88 A/Texas/36/91 A/Taiwan/1/86 A/Leningrad/459/80 A/Hen/Kishinev/75 A/Duck/Alberta/35/76 A/Duck/Ukraine/1/63 A/Duck/California/72
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with the Influenza A virus.
  • Immunogen

    Purified virions.

  • General notes

    Conjugated with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab210526 as a replacement.



Our Abpromise guarantee covers the use of ab20921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/5 - 1/20. Acetone fixation of the antigen in a permissive tissue culture system is recommended prior to direct staining.
Flow Cyt Use at an assay dependent concentration.


  • Relevance
    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • Cellular localization
    Host cell nucleus
  • Alternative names
    • Common flu NP antibody
    • Influenza A virus NP antibody
    • NP antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Protein N antibody
    • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
    see all


  • ab20921 staining Influenza A Virus Nucleoprotein in the Influenza infected Human B-cell lymphoma cell line by Flow Cytometry. Cells were cultured in PBS + 1% BSA and were fixed and permeabilized using a Kit. The sample was incubated with the primary antibody (1/50 in a wash) for 1 hour at 4°C.
    Gating Strategy: Single cell suspension.


This product has been referenced in:
  • Vashist N  et al. Influenza-Activated ILC1s Contribute to Antiviral Immunity Partially Influenced by Differential GITR Expression. Front Immunol 9:505 (2018). Mouse . Read more (PubMed: 29623077) »
  • Perot BP  et al. Autophagy diminishes the early interferon-ß response to influenza A virus resulting in differential expression of interferon-stimulated genes. Cell Death Dis 9:539 (2018). Read more (PubMed: 29748576) »

See all 15 Publications for this product

Customer reviews and Q&As

Gern geschehen, jederzeit gerne wieder!

Vielen Dank für Ihren Anruf.

Wie versprochen habe ich im Labor wegen der Lagerbedingungen für den Anti-Influenza A Virus Nucleoprotein- Antikörper ab20921 nachgefragt, und folgendes wurde uns bestätigt:

Die Instr...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Flow Cytometry
Human Cell (Influenza infected B-cell lymphoma cell line)
Influenza infected B-cell lymphoma cell line
Cell harvesting/tissue preparation method: Spin & Resuspend
Sample buffer: PBS w/ 1% BSA
BD Fix/Perm Kit
Yes - BD Fix/Perm Kit
Gating Strategy
Single cell suspension

Abcam user community

Verified customer

Submitted Oct 20 2011

Thank you for your enquiry. I am sincerely sorry to confirm that we do not have any data to provide regarding the crossreactivity of ab20921 Influenza A Virus Nucleoprotein antibody with Influenza A H1N1 Brisbane/10 or Brisbane 59. This has not...

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