Key features and details
- Mouse monoclonal [9G8] to Influenza A Virus Nucleoprotein
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Influenza A
- Isotype: IgG2a
Product nameAnti-Influenza A Virus Nucleoprotein antibody [9G8]
See all Influenza A Virus Nucleoprotein primary antibodies
DescriptionMouse monoclonal [9G8] to Influenza A Virus Nucleoprotein
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Influenza A
Recombinant full length protein corresponding to Influenza A Virus Nucleoprotein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab43821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/2000 - 1/10000.|
|WB||1/200 - 1/1000.|
|ICC/IF||1/100 - 1/500.
On infected cells
RelevanceThe nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
Cellular localizationHost cell nucleus
- Common flu NP antibody
- Influenza A virus NP antibody
- NP antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab43821 has been referenced in 2 publications.
- Seo YJ et al. Sphingosine kinase 1 serves as a pro-viral factor by regulating viral RNA synthesis and nuclear export of viral ribonucleoprotein complex upon influenza virus infection. PLoS One 8:e75005 (2013). WB . PubMed: 24137500
- Dimmock NJ et al. Comparison of the protection of ferrets against pandemic 2009 influenza A virus (H1N1) by 244 DI influenza virus and oseltamivir. Antiviral Res 96:376-85 (2012). PubMed: 23041142