Anti-Influenza A Virus Nucleoprotein antibody [9G8] (ab43821)

Mouse monoclonal Influenza A Virus Nucleoprotein antibody [9G8]. Validated in WB, ELISA, ICC/IF and tested in Influenza A. Cited in 2 publication(s).

Overview

  • Product name

    Anti-Influenza A Virus Nucleoprotein antibody [9G8]
    See all Influenza A Virus Nucleoprotein primary antibodies
  • Description

    Mouse monoclonal [9G8] to Influenza A Virus Nucleoprotein
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Influenza A
  • Immunogen

    Recombinant full length protein corresponding to Influenza A Virus Nucleoprotein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    9G8
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.
WB 1/200 - 1/1000.
ICC/IF 1/100 - 1/500.

On infected cells 

Target

  • Relevance

    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • Cellular localization

    Host cell nucleus
  • Database links

    • Alternative names

      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all

    References

    This product has been referenced in:

    • Seo YJ  et al. Sphingosine kinase 1 serves as a pro-viral factor by regulating viral RNA synthesis and nuclear export of viral ribonucleoprotein complex upon influenza virus infection. PLoS One 8:e75005 (2013). WB . Read more (PubMed: 24137500) »
    • Dimmock NJ  et al. Comparison of the protection of ferrets against pandemic 2009 influenza A virus (H1N1) by 244 DI influenza virus and oseltamivir. Antiviral Res 96:376-85 (2012). Read more (PubMed: 23041142) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for your response to my further questions. It is very much appreciated.

    When experiments are carried out using transfected cells, it is very important to provide information about the construct and any positive controls used to make sure that the experiments are nicely controlled. Without these details and the exact strains of the virus, it is hard to troubleshoot and to provide sufficient technical support.

    We have not received any similar complaint about ab128193, thus not aware of any QC issues. Currently, we have the same batch in stock your customer has tried. I could offer you a new vial of ab128193 or one vial of any alternative antibodies against the same target protein (Influenza A Virus Nucleoprotein).

    We have the following antibodies which are suitable for Western blot application:

    - https://www.abcam.com/ab43821: Anti-Influenza A Virus Nucleoprotein antibody [9G8]

    - https://www.abcam.com/ab66191: Anti-Influenza A Virus Nucleoprotein antibody [B247M]

    - https://www.abcam.com/ab81126: Anti-Influenza A Virus Nucleoprotein antibody [15]

    Please could you discuss it with your customer and let me know how he/she would like to proceed.

    I look forward to hearing from you soon.

    Read More

    Answer

    Thank you for contacting us.

    I am sorry if ab128193 did not work as you expected.

    H7N3 subtype of avian Influenza A virus has not been tested with this antibody. I would recommend you to test IF with the virus-infected cells or ELISA with inactivated purified antigen of N7H3 to examine the reactivity of C43 antibody with N7H3 subtype.

    In regards to ab43821, it should recognize H7N3 influenza; however, it was not tested on this viral strain.

    You may have received the quotation in a separate email.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    ab43821 has been produced using a recombinant inlfuenza A protein produced in MDCK (Madin-Darby Canine Kidney) cells.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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