Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)


  • Product name
    Anti-Influenza A Virus Nucleoprotein antibody [AA5H]
    See all Influenza A Virus Nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Influenza A
  • Immunogen

    Influenza A/ Puerto Rico/8/34 (H1N1) and A/Bangkok/1/79 (H3N2) viruses.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.5
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >95% pure (SDS-PAGE).
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab20343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/1000.
Flow Cyt Use at an assay dependent concentration. PubMed: 22511783

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • Relevance
    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • Cellular localization
    Host cell nucleus
  • Database links
    • Alternative names
      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all


    • Ab20343 positively staining formaldehyde fixed Influenza A infected human 293T cells (red) counterstained with AUF-1 (green). ab20343 was used at 1/1000 with no antigen retrieval.

      This image is courtesy of an Abreview submitted by Paul Digard on 24 August 2005. We do not have any further information relating to this image.

      See Abreview

    • ab20343 staining Influenza A Virus Nucleoprotein in porcine lung tissue by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
      Host receptor binding assays with H1N1 classical swine strain (A/Sw/Iowa/15/30), a subtype closely related to the human 1918 pandemic influenza virus. Briefly, paraffin embedded 5 µm sections of lung tissues were deparaffinised in xylene and rehydrated by alcohol. Deparaffinised tissue sections were incubated with TPCK trypsin treated swine influenza virus for 24 hours at 37°C. Paradoxically, we found that mammalian H1N1 virus binds more efficiently at 37°C than at the usual 4°C. The sections were washed, blocked with goat serum for 30 minutes, and incubated with ab20343 at a 1/1000 dilution, overnight in a humidified chamber at 4°C. A secondary antibody, FITC-labelled goat anti-mouse IgG was applied at 1/500 dilution for 2 hours at room temperature. After three further washes with TBS, the sections were mounted with ProLong Gold anti-fa
    • Immunocytochemical immunoflurescence analysis of Formaldehyde-fixed human kidney epithelial cells, labelling Influenza A Virus M1 matrix protein with ab20910 at a dilution of 1/500 incubated fro 1 hour at 18°C in 1% FCS PBS. Blocking was with 1% serum incubated for 30 minutes at 18°C. Secondary was a donkey anti-goat Alexa Fluor® 568 undiluted. Cells were transfected with either GFP-M1 or pCDNA2-NP, as indicated on the left hand side of the figure. 24h later cells were fixed and stained for M1 using ab20910 (red) or NP using ab20343 (grey). The abcam 20910 detected M1 in cells transfected with GFP-M1 but not pCDNA3-NP. Moreover, the level of co-localization between GFP-M1 and ab20910 was quite good.

      See Abreview

    • ab20343 used at a 1/200 dilution in Flow Cytometry.
      Ubiquitination negatively regulates antiviral activity of IFITM3.

      A and B, HEK293T cells were transfected overnight with indicated plasmids before a 6-hour infection with influenza virus at a multiplicity of infection of 2.5 and analyzed by flow cytometry. Palm? indicates mutation of Cys-71, Cys-72, and Cys-105 to alanine. Ub? indicates mutation of Lys-24, Lys-83, Lys-88, and Lys-104 to alanine. A, cells expressing IFITM3 constructs were analyzed for the percentage of cells that were infected using ab20343. B, antiviral activity was calculated based on the difference in percentage of infection in HA-IFITM3-positive cells compared with vector control with this value set at 100% antiviral activity. Error bars represent the S.D. of triplicate samples. p values were determined using Student's t test. Data are representative of more than five experiments.


    This product has been referenced in:
    • Tang Y  et al. Antiviral effects of Shuanghuanglian injection powder against influenza A virus H5N1 in vitro and in vivo. Microb Pathog 121:318-324 (2018). IHC-P ; Mouse . Read more (PubMed: 29864534) »
    • Slater T  et al. Bat lung epithelial cells show greater host species-specific innate resistance than MDCK cells to human and avian influenza viruses. Virol J 15:68 (2018). Read more (PubMed: 29636078) »

    See all 49 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us. Your credit note ID is xxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice ...

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    Thank you for taking time to cocontact us and to provide the details of your experiments. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide...

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    Thank you for contacting us.

    Yes, you will need to dilute the antibody when using in applications e.g. IHC, ICC/IF

    Please try concentration10 ug/ml as a start in IHC or ICC/IF.

    I hope this information is helpful to you. Pleas...

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    Thank you for contacting us.

    The product we sell is 200ug per vial. The concentration of lot you have received is 6.87mg/ml which means you should have received a volume of ˜29ul. 4 ul variation could be the pipetting error.

    I hope th...

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    We have one antibody which may be interesting for you: (or use the following: However, please be aware that next to the Puerto Rico (PR)8 strain i...

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    Merci pour votre appel l-autre jour.

    Nous sommes désolés que vous avez reçu un tube qui ne contenait pas l'anticorps liquide. Est-ce que après centrifugation vous avez récupérez un peu de liquide? Read More

    You will not need to dilute your sample any further after eluting from the beads. As the protocol says, you can after boiling load your sample directly onto your gel, without it affecting anything. If there is anything else I can help you with, please...

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    Thanks for your inquiry. The monoclonal antibody to Influenza A Nucleoprotein, catalog ab20343, has been tested and is reactive with the following Influenza A strains: H1N1 A/Puerto Rico/8/34A/USSR/90/77 A/England/333/80 A/Chile/1/83...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Dog Cultured Cells (MDCK)
    Yes - Acetone
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Nov 30 2010

    Thank you for your inquiry. The laboratory confirmed that they have not tested ab20343 against the intact virus.  The following link is to a published reference that describes the development and original characterizaton of this clone.  The paper in...

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