Key features and details
- Mouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein
- Suitable for: ICC/IF, IHC-P, Flow Cyt
- Reacts with: Influenza A
- Isotype: IgG2a
Product nameAnti-Influenza A Virus Nucleoprotein antibody [AA5H]
See all Influenza A Virus Nucleoprotein primary antibodies
DescriptionMouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein
Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cytmore details
Species reactivityReacts with: Influenza A
Influenza A/ Puerto Rico/8/34 (H1N1) and A/Bangkok/1/79 (H3N2) viruses.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notes>95% pure (SDS-PAGE).
Light chain typeunknown
Our Abpromise guarantee covers the use of ab20343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 22511783
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceThe nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
Cellular localizationHost cell nucleus
- Common flu NP antibody
- Influenza A virus NP antibody
- NP antibody
Ab20343 positively staining formaldehyde fixed Influenza A infected human 293T cells (red) counterstained with AUF-1 (green). ab20343 was used at 1/1000 with no antigen retrieval.
This image is courtesy of an Abreview submitted by Paul Digard on 24 August 2005. We do not have any further information relating to this image.
ab20343 staining Influenza A Virus Nucleoprotein in porcine lung tissue by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
Host receptor binding assays with H1N1 classical swine strain (A/Sw/Iowa/15/30), a subtype closely related to the human 1918 pandemic influenza virus. Briefly, paraffin embedded 5 µm sections of lung tissues were deparaffinised in xylene and rehydrated by alcohol. Deparaffinised tissue sections were incubated with TPCK trypsin treated swine influenza virus for 24 hours at 37°C. Paradoxically, we found that mammalian H1N1 virus binds more efficiently at 37°C than at the usual 4°C. The sections were washed, blocked with goat serum for 30 minutes, and incubated with ab20343 at a 1/1000 dilution, overnight in a humidified chamber at 4°C. A secondary antibody, FITC-labelled goat anti-mouse IgG was applied at 1/500 dilution for 2 hours at room temperature. After three further washes with TBS, the sections were mounted with ProLong Gold anti-fa
Immunocytochemical immunoflurescence analysis of Formaldehyde-fixed human kidney epithelial cells, labelling Influenza A Virus M1 matrix protein with ab20910 at a dilution of 1/500 incubated fro 1 hour at 18°C in 1% FCS PBS. Blocking was with 1% serum incubated for 30 minutes at 18°C. Secondary was a donkey anti-goat Alexa Fluor® 568 undiluted. Cells were transfected with either GFP-M1 or pCDNA2-NP, as indicated on the left hand side of the figure. 24h later cells were fixed and stained for M1 using ab20910 (red) or NP using ab20343 (grey). The abcam 20910 detected M1 in cells transfected with GFP-M1 but not pCDNA3-NP. Moreover, the level of co-localization between GFP-M1 and ab20910 was quite good.
ab20343 used at a 1/200 dilution in Flow Cytometry.
Ubiquitination negatively regulates antiviral activity of IFITM3.
A and B, HEK293T cells were transfected overnight with indicated plasmids before a 6-hour infection with influenza virus at a multiplicity of infection of 2.5 and analyzed by flow cytometry. Palm? indicates mutation of Cys-71, Cys-72, and Cys-105 to alanine. Ub? indicates mutation of Lys-24, Lys-83, Lys-88, and Lys-104 to alanine. A, cells expressing IFITM3 constructs were analyzed for the percentage of cells that were infected using ab20343. B, antiviral activity was calculated based on the difference in percentage of infection in HA-IFITM3-positive cells compared with vector control with this value set at 100% antiviral activity. Error bars represent the S.D. of triplicate samples. p values were determined using Student's t test. Data are representative of more than five experiments.
ab20343 has been referenced in 67 publications.
- Kong B et al. Virucidal nano-perforator of viral membrane trapping viral RNAs in the endosome. Nat Commun 10:185 (2019). PubMed: 30643128
- Sun Y et al. Development of a Stable Liquid Formulation for Live Attenuated Influenza Vaccine. J Pharm Sci N/A:N/A (2019). PubMed: 30826350
- Alenquer M et al. Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites. Nat Commun 10:1629 (2019). PubMed: 30967547
- Spence JS et al. IFITM3 directly engages and shuttles incoming virus particles to lysosomes. Nat Chem Biol 15:259-268 (2019). PubMed: 30643282
- Moskovskich A et al. The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection. Sci Rep 9:10471 (2019). PubMed: 31320712