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  1. Link

    influenza-a-virus-nucleoprotein-antibody-aa5h-ab20343.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Influenza
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Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

  • Datasheet
  • SDS
Reviews (2)Q&A (5)References (82)

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Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

Key features and details

  • Mouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Influenza A
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Influenza A Virus Nucleoprotein antibody [AA5H]
    See all Influenza A Virus Nucleoprotein primary antibodies
  • Description

    Mouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Influenza A
  • Immunogen

    Tissue, cells or virus corresponding to Influenza A Influenza A Virus Nucleoprotein. Influenza A/Puerto Rico/8/34 (H1N1) and A/Bangkok/1/79 (H3N2) viruses.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    >95% pure (SDS-PAGE).
  • Clonality

    Monoclonal
  • Clone number

    AA5H
  • Myeloma

    P3x63-Ag8.653
  • Isotype

    IgG2a
  • Light chain type

    unknown
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • single stranded RNA negative strand virus
    • Influenza

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag) (ab224859)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/1000.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/1000.

Target

  • Relevance

    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • Cellular localization

    Host cell nucleus
  • Database links

    • Entrez Gene: 956531 Influenza A
    • SwissProt: P03466 Influenza A
    • SwissProt: P69291 Influenza A
    • Alternative names

      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
      Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

      Ab20343 positively staining formaldehyde fixed Influenza A infected human 293T cells (red) counterstained with AUF-1 (green). ab20343 was used at 1/1000 with no antigen retrieval.

      This image is courtesy of an Abreview submitted by Paul Digard on 24 August 2005. We do not have any further information relating to this image.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)Image from Dr RK Nelli at al, BMC Vet Res. 2010 Jan 27;6:4, Fig 5.
      ab20343 staining Influenza A Virus Nucleoprotein in porcine lung tissue by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
      Host receptor binding assays with H1N1 classical swine strain (A/Sw/Iowa/15/30), a subtype closely related to the human 1918 pandemic influenza virus. Briefly, paraffin embedded 5 µm sections of lung tissues were deparaffinised in xylene and rehydrated by alcohol. Deparaffinised tissue sections were incubated with TPCK trypsin treated swine influenza virus for 24 hours at 37°C. Paradoxically, we found that mammalian H1N1 virus binds more efficiently at 37°C than at the usual 4°C. The sections were washed, blocked with goat serum for 30 minutes, and incubated with ab20343 at a 1/1000 dilution, overnight in a humidified chamber at 4°C. A secondary antibody, FITC-labelled goat anti-mouse IgG was applied at 1/500 dilution for 2 hours at room temperature. After three further washes with TBS, the sections were mounted with ProLong Gold anti-fa
    • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
      Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)Image is courtesy of an AbReview submitted by Dr Maria Amorim.

      Immunocytochemical immunoflurescence analysis of Formaldehyde-fixed human kidney epithelial cells, labelling Influenza A Virus M1 matrix protein with ab20910 at a dilution of 1/500 incubated fro 1 hour at 18°C in 1% FCS PBS. Blocking was with 1% serum incubated for 30 minutes at 18°C. Secondary was a donkey anti-goat Alexa Fluor® 568 undiluted. Cells were transfected with either GFP-M1 or pCDNA2-NP, as indicated on the left hand side of the figure. 24h later cells were fixed and stained for M1 using ab20910 (red) or NP using ab20343 (grey). The abcam 20910 detected M1 in cells transfected with GFP-M1 but not pCDNA3-NP. Moreover, the level of co-localization between GFP-M1 and ab20910 was quite good.

      See Abreview

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (82)

    Publishing research using ab20343? Please let us know so that we can cite the reference in this datasheet.

    ab20343 has been referenced in 82 publications.

    • Pezzotti G  et al. Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses. Sci Rep 11:2977 (2021). PubMed: 33536558
    • Margaroli C  et al. Spatial mapping of SARS-CoV-2 and H1N1 lung injury identifies differential transcriptional signatures. Cell Rep Med 2:100242 (2021). PubMed: 33778787
    • Wu X  et al. Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase. Cell Chem Biol 27:571-585.e6 (2020). PubMed: 32243810
    • Xia S  et al. Coupled CRC 2D and ALI 3D Cultures Express Receptors of Emerging Viruses and Are More Suitable for the Study of Viral Infections Compared to Conventional Cell Lines. Stem Cells Int 2020:2421689 (2020). PubMed: 32695180
    • Wang WC  et al. The Impacts of Reassortant Avian Influenza H5N2 Virus NS1 Proteins on Viral Compatibility and Regulation of Immune Responses. Front Microbiol 11:280 (2020). PubMed: 32226416
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    1-2 of 2 Abreviews

    Immunocytochemistry abreview for Anti-Influenza A Virus Nucleoprotein antibody [AA5H]

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Dog Cultured Cells (MDCK)
    Specification
    MDCK
    Fixative
    Methanol
    Permeabilization
    Yes - Acetone
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 30 2010

    Immunocytochemistry abreview for Anti-Influenza A Virus Nucleoprotein antibody [AA5H]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell lysate - whole cell (influenza A virus infected cells)
    Specification
    influenza A virus infected cells
    Fixative
    Formaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
    Read More

    DR. Paul Digard

    Verified customer

    Submitted Aug 24 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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